高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 15.
• Analytical Sciences • 上一篇 下一篇
ZHAO Jie-Quan1, LIU Bao-Hong1, WU Xing-Liang1, KONG Ji-Lie1, ZOU Yong-Long2, XU Chun-He2
ZHAO Jie-Quan1, LIU Bao-Hong1, WU Xing-Liang1, KONG Ji-Lie1, ZOU Yong-Long2, XU Chun-He2
摘要:
Bio-mimic photosensitive composite films were prepared by linking the monolayer of the photosynthetic reaction centers (RCs) with bifunctional reagents (e.g. 2-mercapto-ethylamine, thiourea) on an Au electrode via Au-S bonding. Square wave voltammetry (SWV), bulk electrolysis, and in-situ CCD spectra were employed for the characterizing the composite films. The composite film behaved reversible or quasi-reversible electron transfer in pH 8.0 tris-HCl buffer. Three peaks were observed centered at 0.468 V,-0.08 V and -0.32 V(vs. NHE) respectively in the potential window from 0.6 V to -0.4 V at low frequency (5-15Hz). Besides, a reproducible peak current fluctuation was observed, i.e. the second and the third peaks disappeared progressively while the first peak increased with the SWV frequency going up, and the electrochemical reaction corresponding to the first peak changed from quasi-reversible to reversible. Three redox couples in RCs, P*/P (the bacteriochlorophyll dimmer, the primary doner), QA-/QA (Primary quinone) and QB-/QB(second quinone) were assigned to contribute to the three reduction peaks respectively. The linear relationship of peak current vs. scanning rate for peak l(i.e. P*/P peak) also showed the reaction of RC in agree with a fast thin-film voltammetric behavior, which implied that the RCs monolayer is linked effectively to the electrode surface.
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