高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 15.

• Analytical Sciences • 上一篇    下一篇

Spectro-Electrochemistry of Photosynthetic Reaction Centers in Coulombically Linked Self-Assembled Monolayers

ZHAO Jie-Quan1, LIU Bao-Hong1, WU Xing-Liang1, KONG Ji-Lie1, ZOU Yong-Long2, XU Chun-He2   

  1. 1. Chem. Dept, Fudan Univ., Shanghai 200433;
    2. Shanghai institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 通讯作者: XU Chun-He
  • 基金资助:

    Projects 39970195、29905001, 39730040 & G1998010100 supported by NSFC.

Spectro-Electrochemistry of Photosynthetic Reaction Centers in Coulombically Linked Self-Assembled Monolayers

ZHAO Jie-Quan1, LIU Bao-Hong1, WU Xing-Liang1, KONG Ji-Lie1, ZOU Yong-Long2, XU Chun-He2   

  1. 1. Chem. Dept, Fudan Univ., Shanghai 200433;
    2. Shanghai institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032
  • Online:2000-12-31 Published:2000-12-31
  • Contact: XU Chun-He
  • Supported by:

    Projects 39970195、29905001, 39730040 & G1998010100 supported by NSFC.

摘要:

Bio-mimic photosensitive composite films were prepared by linking the monolayer of the photosynthetic reaction centers (RCs) with bifunctional reagents (e.g. 2-mercapto-ethylamine, thiourea) on an Au electrode via Au-S bonding. Square wave voltammetry (SWV), bulk electrolysis, and in-situ CCD spectra were employed for the characterizing the composite films. The composite film behaved reversible or quasi-reversible electron transfer in pH 8.0 tris-HCl buffer. Three peaks were observed centered at 0.468 V,-0.08 V and -0.32 V(vs. NHE) respectively in the potential window from 0.6 V to -0.4 V at low frequency (5-15Hz). Besides, a reproducible peak current fluctuation was observed, i.e. the second and the third peaks disappeared progressively while the first peak increased with the SWV frequency going up, and the electrochemical reaction corresponding to the first peak changed from quasi-reversible to reversible. Three redox couples in RCs, P*/P (the bacteriochlorophyll dimmer, the primary doner), QA-/QA (Primary quinone) and QB-/QB(second quinone) were assigned to contribute to the three reduction peaks respectively. The linear relationship of peak current vs. scanning rate for peak l(i.e. P*/P peak) also showed the reaction of RC in agree with a fast thin-film voltammetric behavior, which implied that the RCs monolayer is linked effectively to the electrode surface.

Abstract:

Bio-mimic photosensitive composite films were prepared by linking the monolayer of the photosynthetic reaction centers (RCs) with bifunctional reagents (e.g. 2-mercapto-ethylamine, thiourea) on an Au electrode via Au-S bonding. Square wave voltammetry (SWV), bulk electrolysis, and in-situ CCD spectra were employed for the characterizing the composite films. The composite film behaved reversible or quasi-reversible electron transfer in pH 8.0 tris-HCl buffer. Three peaks were observed centered at 0.468 V,-0.08 V and -0.32 V(vs. NHE) respectively in the potential window from 0.6 V to -0.4 V at low frequency (5-15Hz). Besides, a reproducible peak current fluctuation was observed, i.e. the second and the third peaks disappeared progressively while the first peak increased with the SWV frequency going up, and the electrochemical reaction corresponding to the first peak changed from quasi-reversible to reversible. Three redox couples in RCs, P*/P (the bacteriochlorophyll dimmer, the primary doner), QA-/QA (Primary quinone) and QB-/QB(second quinone) were assigned to contribute to the three reduction peaks respectively. The linear relationship of peak current vs. scanning rate for peak l(i.e. P*/P peak) also showed the reaction of RC in agree with a fast thin-film voltammetric behavior, which implied that the RCs monolayer is linked effectively to the electrode surface.

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