高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 13.

• Analytical Sciences • 上一篇    下一篇

Direct Electrochemical Study of the Redox Species in RC Protein and its Mutants Immobilized in Self-Assembly Monolayers

CAO Yi-Bin1, CHEN Dan-Dan1, WU Xing-Liang1, KONG Ji-Lie1, ZOU Yong-Long2, XU Chun-He2   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433;
    2. Shanghai institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 通讯作者: XU Chun-He
  • 基金资助:

    Projects 39970195、29905001、 39730040 & G1998010100 supported by NSFC.

Direct Electrochemical Study of the Redox Species in RC Protein and its Mutants Immobilized in Self-Assembly Monolayers

CAO Yi-Bin1, CHEN Dan-Dan1, WU Xing-Liang1, KONG Ji-Lie1, ZOU Yong-Long2, XU Chun-He2   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433;
    2. Shanghai institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032
  • Online:2000-12-31 Published:2000-12-31
  • Contact: XU Chun-He
  • Supported by:

    Projects 39970195、29905001、 39730040 & G1998010100 supported by NSFC.

PDF (PC)

被引次数

23

摘要：

4-Aminothiophenol (4-ATP) self-assembled monolayer (SAM) was immobilized on gold electrode. The multi-layered protein film electrode was prepared to rinse the 4-ATP-Au electrode in poly-styrenesulfonate (PSS) and poly-dimerthyldiallylammonium chloride (PDDA) successively, then soaked in a solution containing photosynthetic reaction center (RC) protein from Rhodobacter sphaeriodes or its pigment-replaced mutant. Thus, RC was found embedded in an ordered-orientation film. In cyclic voltammetry (CV) and square wave voltammetry (SWV), Au electrode gave a series of electrochemical signals due to the redox reaction of RC protein or its mutants. Intermolecular direct electron transfer (ET) was studied in this work.

Abstract:

4-Aminothiophenol (4-ATP) self-assembled monolayer (SAM) was immobilized on gold electrode. The multi-layered protein film electrode was prepared to rinse the 4-ATP-Au electrode in poly-styrenesulfonate (PSS) and poly-dimerthyldiallylammonium chloride (PDDA) successively, then soaked in a solution containing photosynthetic reaction center (RC) protein from Rhodobacter sphaeriodes or its pigment-replaced mutant. Thus, RC was found embedded in an ordered-orientation film. In cyclic voltammetry (CV) and square wave voltammetry (SWV), Au electrode gave a series of electrochemical signals due to the redox reaction of RC protein or its mutants. Intermolecular direct electron transfer (ET) was studied in this work.

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