高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (3): 458.

• 论文 • 上一篇    下一篇

含铁离子的XO/HX体系对糖类分子氧化作用的ESR研究

綦丹华1, 罗贵民1, 郑莹光2, 董凤霞2, 杨同书3   

  1. 1. 吉林大学分子酶学工程教育部重点实验室, 长春 130023;
    2. 吉林大学分析测试中心, 长春 130023;
    3. 白求恩医科大学地方病研究所, 长春 130021
  • 收稿日期:1999-04-20 出版日期:2000-03-24 发布日期:2000-03-24
  • 通讯作者: 罗贵民(1943年出生),男,教授,博士生导师,从事分子酶学工程研究.
  • 基金资助:

    国家自然科学基金(批准号:29071013)资助

ESR Spin Trapping Studies on the Radicals Generated from Iron-containing System of Xathine Oxidase and Hypoxathine in the Presence of Saccharide Molecules

QI Dan-Hua1, LUO Gui-Min1, ZHENG Ying-Guang2, DONG Feng-Xia2, YANG Tong-Shu3   

  1. 1. Key Labof Molecular Enzy mologyand Engineeringof Educational Ministry, Jilin University, Changchun 130023;
    2. Analysis&Test Center, Jilin University, Changchun 130023, China;
    3. Instituteof Endemic Diseases, Bethune Medical University, Changchun 130022, China
  • Received:1999-04-20 Online:2000-03-24 Published:2000-03-24

PDF (PC)

被引次数

3

摘要： 用自旋俘获方法直接检测含铁的黄嘌呤氧化酶/次黄嘌呤体系中由糖产生的自由基,发现该体系中除了产生羟基自由基外,还产生烷(氧)基自由基,且糖的浓度越高,体系中产生的烷(氧)基自由基也越多.这表明含铁的黄嘌呤氧化酶/次黄嘌呤体系对糖类分子有氧化作用,且糖的浓度越高,氧化作用越强,对机体的损伤越大.应用含硒抗体酶可有效清除烷(氧)基自由基,显示出很强的抗氧化能力.

关键词: Fe-XO/HX体系, 糖类分子, 烷(氧)基自由基, 自旋俘获, ESR

Abstract: The free radicals generated from iron containing system of xathine oxidase and hypoxathine(Fe XO/HX) were directly detected by using spin trapping. It was found that not only O2-· and OH· but also alkyl or alkoxyl radicals(R· or RO·) were detected when saccharides such as glucose, fructose and sucrose were added into the Fe-XO/HXsystem. The amounts of R· or RO· were increased with the increase of saccharide-concentration added to the Fe-XO/HXsystem. The results showed that saccharide molecules were oxidized by the saccharide containing Fe-XO/HXsystem. R· or RO·has a long life time and they are liposoluble. Thus, they have more toxicity than hydrophilic O2-·and OH·because the active sites of biomolecules are closely related with lipophilic phase. It is worth mentioning that the results have provided an evidence for the hypothesis of "Eating excess sucrose facilitates aging".The R· or RO· radicals were effectively scavenged by selenium-containing abzyme, which displayed a powerful ability to antioxidize.

Key words: Iron-containing xathine oxidase/hypoxathine system, Saccharides, Alkyl/alkoxyl radicals, Spin trapping, ESR

中图分类号: 

TrendMD: