高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (3): 369.

• 论文 • 上一篇    下一篇

流动注射-光度法测定超氧阴离子自由基(O2-·)与超氧化物歧化酶(SOD)活性的研究

杜鸣1, 梁芳珍1, 唐波1, 沈含熙2   

  1. 1. 山东师范大学化学系, 济南 250014;
    2. 南开大学化学系, 天津 300071
  • 收稿日期:1998-05-11 出版日期:1999-03-24 发布日期:1999-03-24
  • 通讯作者: 唐波
  • 作者简介:杜鸣,男,43岁,讲师,博士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29675009);山东省青年自然科学基金

Studies on the Determination of Superoxide Anion Radical(O2-·) and Superoxide Dismudase(SOD) Activity by Flowinjection Spectrophotometry

DU Ming1, LIANG Fang-Zhen1, TANG Bo1, SHEN Han-Xi2   

  1. 1. Department of Chemistry, Shandong Normal University, Jinan, 250014;
    2. Department of Chemistry, Nankai University, Tianjin, 300071
  • Received:1998-05-11 Online:1999-03-24 Published:1999-03-24

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摘要： 合成了4种硫代碳酰腙类化合物双水杨醛硫代碳酰腙(BSTZ)、双对羟基苯甲醛硫代碳酰腙(BHBTZ)、双香兰素硫代碳酰腙(BMHBTZ)和双邻香兰素硫代碳酰腙(BMSTZ),其中苯环醛基对位具有羟基的BHBTZ和BMHBTZ能与超氧阴离子自由基(O2-·)、H2O2发生显色反应,而且BMHBTZ与O2-·的反应灵敏度为最高,由此建立了流动注射-光度法测定超氧阴离子自由基(O2-·)的方法.此方法的线性范围为4.5×10-7～1.5×10-6mol/L,线性方程为A698=1.8×104c(O2-·)+0.0045,相关系数r=0.9984,检出限为1.0×10-7mol/L.相对标准偏差为5.23%.大多数生物样品中共存物质无干扰.

关键词: 硫代碳酰腙类化合物, 超氧阴离子自由基(O2-·, ), SOD活性, 流动注射-光度法

Abstract: Four thiocarbohydrazones of 1,5-bis(salicylidene)thiocarbohydrazone(BSTZ), 1,5-bis(phydroxybenzaldene)thiocarbohydrazone (BHBTZ), 1,5-bis(mmethoxyphydroxybenzaldene)thiocarbohydrazone(BMHBTZ) and 1,5-bis(mmethoxysalicylidene)thiocarbohydrazone(BMSTZ) were synthesized. BHBTZ and BMHBTZ can be oxidated by superoxide anion radical(O2-·) and H2O2.Based on the reaction, the determination of O2-·by flow injection spectrophotometry was developed. The linear range of O2-·concentration is from 4.5×10-7mol/Lto 1.5×10-6mol/L. The detection limit is 1.5×10-7mol/L. The relative standard deviation is 5.23%. Coexist substances in many biological samples have no interference. The method can also be used to determine the activity of superoxide dismudase(SOD).

Key words: Thiocarbohydrazone, Superoxide anion radical, Superoxide dismudase activity, Flow injection spectrophotometry

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