高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (S1): 46.

• Organic Synthesis Chemistry • 上一篇    下一篇

Production and Study of Monoclonal Antiidiotypic Antibody with a Catalytic activity similar to Carboxypeptidase A

DU Mei1, JIN Sheng1, GUO Zheng quan2   

  1. 1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871;
    2. College of Life Science, Peking University, Beijing 100871
  • 出版日期:1998-12-31 发布日期:1998-12-31
  • 基金资助:
    This projcct was supported by National Natural Science Foundation of China (29632004) and the President's Foundation of Peking University

Production and Study of Monoclonal Antiidiotypic Antibody with a Catalytic activity similar to Carboxypeptidase A

DU Mei1, JIN Sheng1, GUO Zheng quan2   

  1. 1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871;
    2. College of Life Science, Peking University, Beijing 100871
  • Online:1998-12-31 Published:1998-12-31
  • Supported by:
    This projcct was supported by National Natural Science Foundation of China (29632004) and the President's Foundation of Peking University

摘要: Catalytic antibody(abzynie) is a new class of biocatalysts. Combining the specificity and the enormous molecular diversity of the antibody with high catalytic efficiency of enzyme to produce catalytic antibody, that is a new method of designing protein. A method of producing catalytic antibody developped recently is to produce antiidiotypic antibody. As the "internal images" of the antigen structure, the antiidiotypic antibody can exhibit the properties similar to the antigen1. To produce the antiidiotypic antibody of the natural enzyme, we may obtain the catalytic antibody whose structure and catalytic activity are similar to the natural euzyme. A. Friboulet et al successfully produced the antiidiotypic catalytic antibody of acetylcholinesterase, whose catalytic activity approaches the natural acetylcholinesterase2. Carboxypeptidase (CPA) has peptidase and esterase activities, to produce the antiidiotypic antibody of CPA, we may obtain the catalytic antibody which catalyze the hydrolysis of amide bond and highly efficiently catalyze the hydrolysis of ester bond. Therefore, this research work is very significant. In this report, we produced a monoclonal antiidiotypic antibody with a catalytic activity similar to the esterase activity of CPA and studied it's kinetic property.

Abstract: Catalytic antibody(abzynie) is a new class of biocatalysts. Combining the specificity and the enormous molecular diversity of the antibody with high catalytic efficiency of enzyme to produce catalytic antibody, that is a new method of designing protein. A method of producing catalytic antibody developped recently is to produce antiidiotypic antibody. As the "internal images" of the antigen structure, the antiidiotypic antibody can exhibit the properties similar to the antigen1. To produce the antiidiotypic antibody of the natural enzyme, we may obtain the catalytic antibody whose structure and catalytic activity are similar to the natural euzyme. A. Friboulet et al successfully produced the antiidiotypic catalytic antibody of acetylcholinesterase, whose catalytic activity approaches the natural acetylcholinesterase2. Carboxypeptidase (CPA) has peptidase and esterase activities, to produce the antiidiotypic antibody of CPA, we may obtain the catalytic antibody which catalyze the hydrolysis of amide bond and highly efficiently catalyze the hydrolysis of ester bond. Therefore, this research work is very significant. In this report, we produced a monoclonal antiidiotypic antibody with a catalytic activity similar to the esterase activity of CPA and studied it's kinetic property.

TrendMD: