高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (S1): 2.

• Plenary, Keynote and Invited Lectures • 上一篇    下一篇

The Development of "Caged" Substrates for the Studies of Rapid Electron Transfer and Early Events in Protein Folding

Sunney I. Chan   

  1. Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan;and Noyes Laboratory, California Institute of Technology, Pasadena, CA 91125, U. S. A
  • 出版日期:1998-12-31 发布日期:1998-12-31

The Development of "Caged" Substrates for the Studies of Rapid Electron Transfer and Early Events in Protein Folding

Sunney I. Chan   

  1. Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan;and Noyes Laboratory, California Institute of Technology, Pasadena, CA 91125, U. S. A
  • Online:1998-12-31 Published:1998-12-31

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摘要： In recent years,the applications of photolabile compounds, or compounds that unmask a function group upon absorption of light, have become more diverse. Since such compounds can provide an effective means of orthogonal protection, they have been used to facilitate the synthesis of complex, polyfunctional organic molecules. These materials have also been used to "cage" compounds by protection of an essential functional group, so that a chemical reaction may be initiated by a pulse of light. In this manner, mixing difficulties can be circumvented in kinetics measurements. Applications of this method include the photolysis of caged ATP for studies of muscle fiber contraction, where diffusion of ATP into the muscle fiber is, slow; of caged fluorescent probes that only emit light after photolysis; and even of caged enzymes by incorporating photolabile groups on essential side chains.

Abstract: In recent years,the applications of photolabile compounds, or compounds that unmask a function group upon absorption of light, have become more diverse. Since such compounds can provide an effective means of orthogonal protection, they have been used to facilitate the synthesis of complex, polyfunctional organic molecules. These materials have also been used to "cage" compounds by protection of an essential functional group, so that a chemical reaction may be initiated by a pulse of light. In this manner, mixing difficulties can be circumvented in kinetics measurements. Applications of this method include the photolysis of caged ATP for studies of muscle fiber contraction, where diffusion of ATP into the muscle fiber is, slow; of caged fluorescent probes that only emit light after photolysis; and even of caged enzymes by incorporating photolabile groups on essential side chains.

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