Chem. J. Chinese Universities

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Simultaneous Determination of Sudan Ⅱ and Sudan Ⅲ via Synchronous Fluorescence Spectrometry

CAI Qi-Hong1,2, ZOU Zhe-Xiang1, LI Yao-Qun1*   

    1. College of Chemistry and Chemical Engineering, Key Laboratory of Analytical Sciences of MOE, Xiamen University, Xiamen 361005 , China;
    2. Department of Pharmacy, Putian College, Putian 351100 , China
  • Received:2006-12-22 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10
  • Contact: LI Yao-Qun

Abstract: A new method of constant-wavelength synchronous fluorescence spectrometry for simultaneous determination of Sudan Ⅱand Sudan Ⅲ was developed with using their fluorescence phenomenon. Only one single scan is needed for effective identification and quantitative determination of the two compounds simultaneously when Δλ=60 nm is chosen. The linear ranges for Sudan Ⅱ and Sudan Ⅲ were 0—3.3 and 0—2.8 μg/mL, respectively. The detection limits for Sudan Ⅱ and Sudan Ⅲ were 14 and 11 ng/mL, respectively. By using this method, Sudan Ⅱand Ⅲ in sausage samples were determined directly with the recoveries of 83.8%—109.5%.

Key words: Sudan Ⅱ, Sudan Ⅲ, Fluorescence spectrometry, Synchronous scanning

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