CJCU

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (1): 71.

• Articles • Previous Articles     Next Articles

A Mathematical Model for Biosynthesis of Medium-chain-length Polyhydroxyalkanoates by Pseudomonas stutzeri1317

XU Jun1, HE Wen-Nan1, GUO Bao-Hua1, HU Ping1, ZHANG Zeng-Min1, ZHANG Guang2, LIN Hui-Lan2, CHEN Jin-Chun2, CHEN Guo-Qiang2   

  1. 1. Institute of Polymer Science & Engineering, Department of Chemical Engineering, School of Materials Science and Engineering, Tsinghua Unviversity, Beijing 100084, China;
    2. Department of Biological Science & Technology, Tsinghua University, Beijing 100084, China
  • Received:2000-06-27 Online:2002-01-24 Published:2002-01-24

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Abstract: When grown on various carbon sources, P. stutzeri 1317 strain accumulates medium-chain-length polyhydroxyalkanoates(mcl PHAs) of various monomer composition. Amathematical model was developed to investigate what affects mcl PHAcomposition and how to tailor design the copolymer, and some interesting conclusions were deduced: the monomer composition was independent of cultivation time and the enzyme level; the content variance of different monomers resulted only from the selectivity ratio of the polymerase to the enzymes involved in theprecursor formation pathway; modulation of precursor formation pathway led to convenient manipulation of PHA monomer composition. The experimental results from several Pseudomonas strains proved the model an effective tool for tailor-design of mcl PHA copolymers.

Key words: Medium-chain-length polyhydroxyalkanoates(mcl PHAs), Pseudomonas stutzeri, Mathematical model, Monomer composition, Metabolic control analysis

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