ESR Spin Trapping Studies on the Radicals Generated from Iron-containing System of Xathine Oxidase and Hypoxathine in the Presence of Saccharide Molecules

Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (3): 458.

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ESR Spin Trapping Studies on the Radicals Generated from Iron-containing System of Xathine Oxidase and Hypoxathine in the Presence of Saccharide Molecules

QI Dan-Hua¹, LUO Gui-Min¹, ZHENG Ying-Guang², DONG Feng-Xia², YANG Tong-Shu³

1. Key Labof Molecular Enzy mologyand Engineeringof Educational Ministry, Jilin University, Changchun 130023;
2. Analysis&Test Center, Jilin University, Changchun 130023, China;
3. Instituteof Endemic Diseases, Bethune Medical University, Changchun 130022, China

Received:1999-04-20 Online:2000-03-24 Published:2000-03-24

Abstract

Abstract: The free radicals generated from iron containing system of xathine oxidase and hypoxathine(Fe XO/HX) were directly detected by using spin trapping. It was found that not only O₂^-· and OH· but also alkyl or alkoxyl radicals(R· or RO·) were detected when saccharides such as glucose, fructose and sucrose were added into the Fe-XO/HXsystem. The amounts of R· or RO· were increased with the increase of saccharide-concentration added to the Fe-XO/HXsystem. The results showed that saccharide molecules were oxidized by the saccharide containing Fe-XO/HXsystem. R· or RO·has a long life time and they are liposoluble. Thus, they have more toxicity than hydrophilic O₂^-·and OH·because the active sites of biomolecules are closely related with lipophilic phase. It is worth mentioning that the results have provided an evidence for the hypothesis of "Eating excess sucrose facilitates aging".The R· or RO· radicals were effectively scavenged by selenium-containing abzyme, which displayed a powerful ability to antioxidize.

Key words: Iron-containing xathine oxidase/hypoxathine system, Saccharides, Alkyl/alkoxyl radicals, Spin trapping, ESR

CLC Number:

Q53
O433.4

TrendMD:

QI Dan-Hua, LUO Gui-Min, ZHENG Ying-Guang, DONG Feng-Xia, YANG Tong-Shu. ESR Spin Trapping Studies on the Radicals Generated from Iron-containing System of Xathine Oxidase and Hypoxathine in the Presence of Saccharide Molecules[J]. Chem. J. Chinese Universities, 2000, 21(3): 458.

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ESR Spin Trapping Studies on the Radicals Generated from Iron-containing System of Xathine Oxidase and Hypoxathine in the Presence of Saccharide Molecules

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