Abstract: To purify the enzymolysis product of porcine blood by trypsin hydrolysis via the biological technique such as ultra-filter and chromatography, we obtain the aim product using HPLC to determine the purity of our aim peptide, its molecular weight is about 3 000. In order to identify the existence of our aim peptide, we performed T lymophocyte transformation test and E rosette forming test for the product of the progress. Furthermore, we carried out the experiments of immunology and pharmacology in vivo and in vitro to ensure that our aim peptide can prove the enhancement immunologic activity of organism.

Key words: Bioactive peptide, T lymphocyte transformation test, Pig blood hydrolyzed by trypsin