Abstract: Schizandrin A, which has been shown to have anticarcinogenic activity and hepatoprotective effect is the active schisandra lignan from a traditional chinese medicine schisandra chinensis (wuweizi). A novel method for quantitative analysis of schizandrin A in human plasma samples, urine samples and cell culture media was proposed by combining excitation-emission matrix fluorescence spectra with second-order calibration method based on self-weighted alternating trilinear decomposition algorithm (SWATLD). Satisfactory results have been achieved, fully exploiting “second-order advantage”. The average recoveries of schizandrin A in human plasma samples, urine samples and cell culture media obtained by using SWATLD with N=2 are （100.4±1.6）%，（100.5±6.3）% and（103.6±4.5）%, respectively. It proved that SWATLD based second-order calibration methodologies can be feasible to directly quantify the content of schizandrin A in human plasma samples, urine samples and cell culture media. It could be applied to quantitative analysis of schizandrin A even in the presence of unknown interferences, without time-consuming and complicated pretreatment.

Key words: Schizandrin A, Excitation-emission matrix fluorescence, Self-weighted alternating trilinear decomposition algorithm(SWATLD), Second-order calibration