CJCU

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 800.

• Articles • Previous Articles     Next Articles

Preparation, Characterization of PAMAM Dendrimers-coated Liposomes and Evaluation for Ophthalmic Drug Delivery

LIU Yi, SUN Kao-Xiang*, YAO Wen-Jun, LIANG Na, MU Hong-Jie, LIANG Rong-Cai, YAO Chen   

  1. School of Pharmacy, Yantai University, Yantai 264005, China
  • Received:2009-07-20 Online:2010-04-10 Published:2010-04-10
  • Contact: SUN Kao-Xiang. E-mail: sunkaoxiang@luye.cn
  • Supported by:

    国家自然科学基金(批准号: 30873447)资助.

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Abstract:

PAMAM dendrimers-coated liposomes encapsulated puerarin were prepared and the particle size, zeta potential and drug release property were investigated. Fluorescein isothiocyanate (FITC)-labeled PAMAM dendrimers were prepared to study adsorption rate. Transmission electron microscope and laser confocal scanning microscopy were used to explore the morphology and structure of PAMAM-coated liposomes. The in vitro transcorneal penetration, precorneal residence time, residual amount of puerarin within corneal of PAMAM coated liposomes were evaluated. The results showed that the size of the coated liposomes was little larger than that of the uncoated liposome but no significantly difference and the zeta potential became positive from negative and increased along with ratio of PAMAM. The cumulative release of PAMAM coated liposomes was similar to that of uncoatd liposome with slower release property. The PAMAM coated liposomes were nonirritant and could remarkably improve permeability of puerarin transcornea, prolong corneal residence time, increase residual amount of PUE within cornea, compared with uncoated liposome and PUE drop solution(P＜0.01). The PAMAM coated liposomes is promising as a novel ophthalmic drug delivery system.

Key words: Dendrimer; Poly(amidoamine); Puerarin; Coated liposome; Transcorneal penetration

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