Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (2): 303.

• Articles • Previous Articles     Next Articles

Preparation of Series ι-Carrageenan Oligosaccharides and Sequence Determination by Negative-ion Electrospray Tandem Mass Spectrometry

YANG Bo, YU Guang-Li*, HAO Cui, ZHAO Xia, JI Guo-Li, HU Yan-Nan, WANG Yu-Feng   

  1. Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • Received:2009-03-10 Online:2010-02-10 Published:2010-02-10
  • Contact: YU Guang-Li. E-mail: glyu@ouc.edu.cn
  • Supported by:

    国际科技合作专项基金(批准号: 2007DFA30980)、国家海洋“八六三”计划专项基金(批准号: 2007AA09Z445)和国家自然科学基金(批准号: 30870506)资助.

Abstract:

Ten oligosaccharides were separated from ι-carrageenan mild acid hydrolysate with low pressure gel permeation chromatography(LPGPC). Based on the purity analysis of polyacrylamide gel electrophoresis(PAGE) and high performance thin layer chromatography(HPTLC), the structures of those oligosaccharides were determined by infrared spectrum(IR), nuclear magnetic resonance spectroscopy(NMR) and electrospray mass spectrometry(ESI-MS). Their sequences were confirmed as ι-carrageenan disaccharide to eicosasaccharide with 4-linked-2-O-sulfated-α-D-3,6-anhydrogalactose(A2S) at the reducing and 3-linked-4-O-sulfated-β-D-galactopyranose(G4S) at the non-reducing terminus by electrospray collision-induced dissociation mass spectrometry(ESI-CID-MS/MS). The results show that the structural feature of these ι-carr-oligosaccharides from mild acid hydrolysis is completely different from those neo-ι-carra-oligosaccharides prepared by ι-carra-geenase digestion. All these ι-carrageenan derived oligosaccharides enrich marine carbohydrate library. More importantly, they provide the foundation for the preparation of the oligosaccharide-chip and the investigation on the interaction between oligosaccharide and protein.

Key words: ι-Carrageenan oligosaccharide; Separation and purification; ESI-CID-MS/MS; Sequence analysis

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