CJCU

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (4): 646.

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Interaction of  Glyceraldehyde-3-phosphate Dehydrogenase in S.cerevisiae with Mitochndrial Genome
Maintenance Protein Mgm101

XUE Dong-Hua 1,2, WANG Li 1* ,  ZUO Xiao-Ming3   

  1. 1. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;
     2. School of Biological Engineering,Changchun University of Technology, Changchun 130012, China;
    3. Molecular Genetics and Evolution Group, Research School of Biological Science,
    the Australian National University, Canberra ACT 2601, Australia
  • Received:2005-09-06 Online:2006-04-10 Published:2006-04-10
  • Contact: WANG Li, wangli@nenu.edu.cn

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Abstract:

MGM101 is involved in mitochondrial genome maintenance in yeast. Mgm101p is a protein required for the replication of mtDNA. By means of yeast two-hybrid, we screened a Schizosaccharomyces pombe cDNA library for clones encoding proteins that potentially interact with Mgm101p. We have isolated 15 mt-DNA-associated proteins in yeast. We had identified five individual clones with GPD1 coding for glyceraldehyde 3-phosphate dehydrogenase(GAPDH). The S. cerevisiae counterpart of the GPD1, TDH2 was cloned and examined for its possibility to be involved in mitochondrial genome maintenance. GFP tagging, subcellular fractionation followed by Western blotting analysis revealed that GAPDH, besides having a cytoplasmic location, was imported into mitochondria and had potential to interact with Mgm101p. These results suggest that GAPDH is likely a multifunctional protein and interacts with Mgm101p  in mitochondria, which is involved in the process mediated by mtDNA.

Key words: Glyceraldehyde-3-phosphate dehydrogenase; mtDNA; Yeast two-hybrid; Mgm101 protein

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