Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (11): 2180.

• Articles • Previous Articles     Next Articles

Dot Immunofiltration Assay for Quantitative Detecting cTnI Using Quantum Dots

FAN Jia1,3, SONG Jian2, BI Li-Rong5, ZHOU Guang-Yu5, ZHANG Hao4, WEI Jing-Yan1,3*, YANG Bai4   

  1. 1. College of Pharmacy,
    2. College of Electronic Science and Engineering,
    3. Key Laboratory of Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, China;
    4. College of Chemistry, Jilin University, Changchun 130012, China;
    5. China-Japan Union Hsopital, Jilin University, Changchun 130033, China
  • Received:2008-11-24 Online:2009-11-10 Published:2009-11-10
  • Contact: WEI Jing-Yan. E-mail: jingyanwei@yahoo.com.cn
  • Supported by:

    吉林省科学技术厅基金(批准号: 200705380)、中国博士后基金(批准号: 2004036395)和吉林大学本科生创新性实验计划(批准号: 2007C3268)资助.

Abstract:

Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with ImageMaster to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R2=0.9966).

Key words: Cardiac troponin I; Monoclonal antibody to cardiac troponin I; Dot immunofiltration assay; Quantum dot

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