A Fluorescent Probe for Detection of Microcystin-LR Based on Poly(thymidine)-Copper Nanoclusters and Aptamer-Gold Nanoparticles

doi:10.7503/cjcu20250008

Chem. J. Chinese Universities

• Article • Previous Articles Next Articles

A Fluorescent Probe for Detection of Microcystin-LR Based on Poly(thymidine)-Copper Nanoclusters and Aptamer-Gold Nanoparticles

MENG Hua¹，A Huaying¹，LUO Pingxin¹，ZHANG Yanli¹，GAO Lianxun¹，WANG Hongbin¹， YANG Wenrong^1,2，PANG Pengfei¹

1. Yunnan Minzu University 2. School of Life and Environmental Sciences, Deakin University

Received:2025-01-08 Revised:2025-02-19 Online:2025-03-05 Published:2025-03-05
Contact: Pengfei Pang E-mail:pfpang@aliyun.com
Supported by:
Supported by the National Natural Science Foundation of China (Nos. 21665027, 21565031), Basic Research Program Project of Yunnan Provincial Science and Technology Department, China (No. 202001AT070012), and Graduate Research Innovation Fund Project of Yunnan Minzu University, China (No. 2024SKY135)

1. ESM to 20250008.pdf(754KB)

Abstract

Abstract: A fluorescent probe of poly(thymidine)-copper nanoclusters/aptamer-gold nanoparticles (poly(T)- CuNCs/aptamer-AuNPs) was constructed for highly sensitive sensing detection of microcystin-LR (MC-LR) using DNA template method. Three DNA nucleotides were designed, including MC-LR aptamer, and two poly(thymidine) ssDNA (poly(T) S1 and poly(T) S2). Using poly(T) S1 and poly(T) S2 as templates, poly(T) S1-CuNCs and poly(T) S2-CuNCs with pink fluorescence were synthesized by reduction Cu2+ with ascorbic acid (AA). Aptamer labeled with thiol groups at two both ends, were linked with AuNPs through Au-S bonds to form AuNPs-aptamer-AuNPs bioconjugates. AuNPs-aptamer-AuNPs hybridized with poly(T)-CuNCs to form dsDNA-CuNCs. Fluorescence resonance energy transfer (FRET) occurred between CuNCs and AuNPs in the dsDNA-CuNCs structure, leading to fluorescence quenching of dsDNA-CuNCs. In the presence of target MC-LR, MC-LR specifically bonded with aptamer in dsDNA-CuNCs, resulting in the dissociation of dsDNA structure. The poly(T)-CuNCs were released into the solution, restoring the system fluorescence. An "off-on" type of fluorescent probe was constructed for detection of MC-LR. The linear range for MC-LR detection is 1 ng/L ~ 500 μg/L, with a detection limit of 0.3 ng/L (S/N = 3). The proposed fluorescent aptamer probe possesses the advantages of simple preparation, high selectivity, and can be applied for quantitative detection and analysis of MC-LR in real water samples.

Key words: Microcystin-LR；Poly(thymine)-copper nanoclusters；Aptamer-gold nanoparticles；Fluorescent probe

CLC Number:

O657.3

TrendMD:

MENG Hua, A Huaying, LUO Pingxin, ZHANG Yanli, GAO Lianxun, WANG Hongbin, YANG Wenrong, PANG Pengfei. A Fluorescent Probe for Detection of Microcystin-LR Based on Poly(thymidine)-Copper Nanoclusters and Aptamer-Gold Nanoparticles[J]. Chem. J. Chinese Universities, doi: 10.7503/cjcu20250008.

[1]	WU Xiaoyu, ZHU Feng, KUANG Yuanheng, LIU Bohua, SUN Chongling, WANG Yanyan. Fabrication of AuNRs-PDMS Micropillar Structure and Its Label-free SERS Detection for Regulated Living Cells [J]. Chem. J. Chinese Universities, 2025, 46(3): 20240364.
[2]	CHEN Shan, ZHUO Yufei, LI Jingying. Nucleic acid-based protein labeling tools for visualization of membrane receptors [J]. Chem. J. Chinese Universities, 0, (): 20240567.
[3]	WANG Changying, ZHANG Dawei, CHEN Guanji, ZHANG Zhenwei, XIAO Weihong, WANG Bin, CHEN Qidan, YANG Bai. Preparation of carbon dots fluorescent marker and the application in NO2- specific detection [J]. Chem. J. Chinese Universities, 0, (): 20240519.
[4]	BI Yingna, LIU Dingbin. Internal Standard Method-based Surface-enhanced Raman Spectroscopy for Quantitative Analysis [J]. Chem. J. Chinese Universities, 2025, 46(2): 20240457.
[5]	YANG Qian, Tu Peng, HAO Zhenfang, CUI Yanjun, BIAN Hongxia. Monitoring of ethylene release from plant tissues by fluorescent probe and application in white ripe tomato [J]. Chem. J. Chinese Universities, 0, (): 20240517.
[6]	WANG Zhiyuan, DONG Yi, QI Baohui, WEI Xueyang, ZHANG Jiahui, HUANG Qizhong, LI Jisheng, GAO Na, DI Shiying, Hu Yufeng. Determination of Acidic Ionic Liquid H0 and the Influence of Salt Effect [J]. Chem. J. Chinese Universities, 0, (): 20240535.
[7]	WANG Chao, JIA Aiping, ZHANG Yawen, CHEN Guang. 3，4-Dimethoxychalcone Fluorescent Probe for the Detection of Human Serum Albumin [J]. Chem. J. Chinese Universities, 2024, 45(10): 20240273.
[8]	GONG Wenpeng, ZHOU Linnan. Fluorescent Detection of Hydrogen Sulfide Using Metal-organic Framework CAU-10-NH-DNBA Functionalized with 3，5-Dinitrobenzoic Acid [J]. Chem. J. Chinese Universities, 2024, 45(8): 20240069.
[9]	LIU Xuan, CHEN Qipei, OU Jingmei, HOU Jian. Solid Tunable Ratiometric Fluorescence Thermometers Based on Triarylboron-Phase Change Materials [J]. Chem. J. Chinese Universities, 2024, 45(8): 20240189.
[10]	CHEN Qidan, CHEN Guanji, YOU Shanmei, ZANG Xinyao, YANG Bai. Preparation of broad-spectrum UV blocking carbon dots for the application of sunscreen absorbent [J]. Chem. J. Chinese Universities, 0, (): 20240313.
[11]	LI Yin, LIU Jinsong, WANG Yu, QI Tingting, MENG Leyuan, ZHANG Jing, LYU Dongmei, JIAO Huping. Direct Evaluation of Single-strand Break Repair Capacity of Cells Using a Pyrene Excimer-based Hairpin Probe [J]. Chem. J. Chinese Universities, 2023, 44(11): 20230288.
[12]	FU Fangmei, XU Mengru, LIANG Zishan, HUANG Sirui, LI Hui, ZHANG Haoran, LI Wei, ZHENG Mingtao, LEI Bingfu. Conjugate Size and Surface Oxidation Synergistically Trigger Red Fluorescence in Carbon Dots for Detecting Trace Water in Organic Solvents [J]. Chem. J. Chinese Universities, 2023, 44(2): 20220464.
[13]	WANG Di, ZHONG Keli, TANG Lijun, HOU Shuhua, LYU Chunxin. Synthesis of Schiff-based Covalent Organic Framework and Its Recognition of I ^‒ [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220115.
[14]	WANG Junyang, LIU Zheng, ZHANG Qian, SUN Chunyan, LI Hongxia. Application of DNA Silver Nanoclusters in the Fluorescence Biosensors based on Functional Nucleic Acids [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220010.
[15]	CHEN Jiamin, QU Xiaozhang, QI Guohua, XU Weiqing, JIN Yongdong, XU Shuping. SERS Nanoprobe for the Detection of Reactive Oxygen Species in Cells Produced by Electrostimulus [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220033.

A Fluorescent Probe for Detection of Microcystin-LR Based on Poly(thymidine)-Copper Nanoclusters and Aptamer-Gold Nanoparticles

PDF (PC)

Supplement

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments