Preparation of Microcapsular Polycaprolactone Nanofibers by Emulsion Electrospinning and Their Protein Encapsulation

doi:10.7503/cjcu20230199

Abstract

Abstract:

The microcapsular polycaprolactone（PCL） nanofibers were prepared by water-in-oil emulsion electrospinning as the carrier for protein encapsulation. The effects of ultrasonic emulsification， water addition amount， surfactant concentration and aqueous phase additives on the morphology of microcapsules in emulsion were investigated. The optimal preparative conditions to achieve the long-term stable polymer emulsion for nanofiber membrane are as follows： the dosage of amphiphilic triblock copolymer（Pluronic^® F108） as surfactant was 30%（mass fraction） in 10%（mass fraction） PCL spinning liquid， the oil-to-water volume ratio was 9∶1 and the aqueous phase was added extra glycerol and dopamine as additives. The properties of nanofibers were characterized by scanning electron microscopy， pore size distribution determination， etc. The results showed that the surface area of nanofiber was 31.784 m²/g， the internal microcapsule size distributed around 1.06 µm， and the load of bovine serum albumin（BSA） could reach 12.89 mg/g. Further， the release behavior of encapsulated BSA in nanofiber was studied. When the aqueous phase additives were added， the release rate of BSA was lower to 10.03% after 4 h by Coomassie blue staining test. The immobilization rate of protein was as high as 89.97%. In addition， the fluorescent labeled BSA was observed to be concentrated in the internal microcapsular structures of nanofiber verified by confocal laser scanning microscopy. The above characterization and analysis showed that a novel microcapsular structure of nanofiber for protein encapsulation was fabricated by emulsion electrospinning， which is different from the conventional hollow/core-shell structure and has a greater prospect of application in enzyme immobilization and biocatalysis.

Key words: Electrospinning, Nanofiber, Water-in-oil emulsion, Microcapsule, Encapsulated protein

CLC Number:

O648.2

TrendMD:

GAO Chong, ZHOU Quan, YANG Fan, REN Ruipeng, LYU Yongkang. Preparation of Microcapsular Polycaprolactone Nanofibers by Emulsion Electrospinning and Their Protein Encapsulation[J]. Chem. J. Chinese Universities, 2023, 44(10): 20230199.

Figures/Tables 8

References 38

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