Sensitive Detection of L⁃Arginine Using Peptide Aptamer Functionalized Nanochannel

doi:10.7503/cjcu20220758

Chem. J. Chinese Universities ›› 2023, Vol. 44 ›› Issue (6): 20220758.doi: 10.7503/cjcu20220758

• Analytical Chemistry • Previous Articles Next Articles

Sensitive Detection of L⁃Arginine Using Peptide Aptamer Functionalized Nanochannel

CAO Zhong¹, GUO Peixian¹, XUE Shulei¹, XIAO Zhongliang¹, TAN Shuqi¹, KANG Xuan¹, FENG Zemeng², YIN Yulong², MA Tianji¹()

^1.Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation，Hunan Provincial Key Laboratory of Cytochemistry，School of Chemistry and Chemical Engineering，Changsha University of Science and Technology，Changsha 410114，China
^2.Institute of Subtropical Agriculture，Chinese Academy of Sciences，Changsha 410125，China

Received:2022-12-13 Online:2023-06-10 Published:2023-02-14
Contact: MA Tianji E-mail:mtjbj2008@163.com
Supported by:
the National Natural Science Foundation of China(22204007);the Scientific Research Fund of Hunan Provincial Education Department, China(21A0182)

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Abstract

Abstract:

Amino acids are the source of life， in which L⁃arginine（L⁃Arg） is not only an important amino acid for metabolism in organisms， but also one of the important tumor markers. It is very important to develop highly selective methods for the detection of L⁃Arg in the field of bioanalysis. The polypeptide sequence specifically binding to L⁃Arg （sequence CFGHIHEGY） was screened from 29185 protein sequences in Uniprot database and was immobilized on the tip surface of bullet shaped nanochannel as a recognition element. Under the nanoconfined effect， the conformational change from extended state to curled state before and after the specific binding of peptides to L⁃Arg was used to control the effective pore size of nanochannel， resulting in the change of nanopore conductivity， which realize the selective detection of L⁃Arg. The experimental results show that the polypeptide modified nanochannel has high sensitivity and selectivity for L⁃Arg， the linear range is 1—100 nmol/L， and the limit of detection is as low as 1 nmol/L. This research provides a new method for sensible and highly selective amino acid sensing and also provides a new idea for designing peptide functionalized bio-inspired ionic channel.

Key words: Nanopore, Ionic channel, Nanoconfined effect, Peptide aptamer, Detection of L?arginine

CLC Number:

O657

TrendMD:

CAO Zhong, GUO Peixian, XUE Shulei, XIAO Zhongliang, TAN Shuqi, KANG Xuan, FENG Zemeng, YIN Yulong, MA Tianji. Sensitive Detection of L⁃Arginine Using Peptide Aptamer Functionalized Nanochannel[J]. Chem. J. Chinese Universities, 2023, 44(6): 20220758.

Figures/Tables 5

References 32

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Sensitive Detection of L⁃Arginine Using Peptide Aptamer Functionalized Nanochannel

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