Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1965.

• Articles • Previous Articles     Next Articles

Protein Expression Alternations for the HaCaT Proteome in Response to the UVB-irradiation and Chemical-carcinogen

SUN Ming-Zhong1*, LIU Shu-Qing2*, LÜ Xiao2, GUO Yi-Meng1, GUO Chun-Mei1, XIN Yi1, TANG Jian-Wu3   

  1. 1. Department of Biotechnology,
    2. Department of Biochemistry and Molecular Biology,
    3. Department of Pathology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China
  • Received:2008-08-27 Online:2009-10-10 Published:2009-10-10
  • Contact: SUN Ming-Zhong, E-mail: lsqsmz@gmail.com; LIU Shu-Qing, E-mail: mxs288@gmail.com
  • Supported by:

    国家自然科学基金(批准号: 30572098)和大连医科大学科研启动经费(批准号: 2007001, 2007003)资助.

Abstract:

The keratinocyte cell line, HaCaT, was radiated by UVB-irradiation, induced by the 2,4-dinitrobenzene sulfonic acid(DNBS) and stimulated by the combination of UVB-irradiation and DNBS-stimulation(UD), respectively. The differentially expressed protein candidates in response to those stimulations were se-parated by two-dimensional fluorescent in gel electrophoresis(2D DIGE), and the expression levels were quantified by DeCyder software and identifications of interested proteins were performed by the high perfor-mance liquid chromatography nano electrospray tandem mass spectrometry(HPLC-nESI-MS/MS) analysis. Sixty-five protein spots were found differentially expressed with metastatic significance(P<0.05). Comparing to the HaCaT cell treated either by UVB alone or DNBS alone, forty-one and thirteen protein spots were shown positively cooperatively regulated and negatively cooperatively regulated by the stimulations of UVB plus DNBS, respectively; and there are five and six protein spots show similar expression levels as the cells being treated with UVB alone and stimulated with DNBS alone. Sixty unique proteins were identified from all the sixty-five protein spots by HPLC-nESI-MS/MS analysis. Those protein candidates were systemically categorized by bioinformatics analysis based on their molecular functions, involvements in biological processes and pathways. The results from current study provide the protein candidates directly associated with the UVB irradiation and chemical stimualtion, which helps to investigate the formation mechanisms of skin cancers under different environmental conditions and to prevent and treat skin diseases.

Key words: HaCaT cell line; UVB irradiation; 2,4-Dinitrobenzene sulfonic acid; Fluorescence two-dimensional difference in-gel electrophoresis

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