Screening of Xanthine Oxidase Inhibitors in Traditional Chinese Herbal Formulae Ermiaowan Using Ultrafiltration UPLC-MSn†

doi:10.7503/cjcu20140419

Abstract

Abstract:

An ultrafiltration UPLC/Q-TOF/MS assay and in vitro xanthine oxidase inhibition assay were combined to screen xanthine oxidase inhibitors in traditional Chinese herbal formulae Ermiaowan. The result of in vitro xanthine oxidase inhibition assay showed that Ermiaowan had a strong xanthine oxidase inhibitory activity, the IC₅₀ value was (0.218±0.0034) mg/mL. Nine compounds were screened and identified as potential xanthine oxidase inhibitors in Ermiaowan using ultrafiltration UPLC/Q-TOF/MS. This work provides a certain basis for the development of xanthine oxidase inhibitors and the mechanism clarification of Ermiaowan to treat gout and hyperuricemia.

Key words: Ermiaowan, Xanthine oxidase, Ultrafiltration UPLC/Q-TOF/MS

CLC Number:

O657.6

TrendMD:

XU Chen, LIU Shu, LIU Zhiqiang, SONG Fengrui. Screening of Xanthine Oxidase Inhibitors in Traditional Chinese Herbal Formulae Ermiaowan Using Ultrafiltration UPLC-M $S n †$ [J]. Chem. J. Chinese Universities, 2014, 35(8): 1640.

Figures/Tables 8

References 20

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Compound	t_R/min	Formula	Measured, m/z	Theory, m/z	10⁶ Error	MS², m/z	Binding degree(%)
Phellodendrine(1)	5.2	C₂₀H₂₄NO₄	342.1698	342.1705	-2.0	192.10	3.25
Magnoflorine(2)	6.2	C₂₀H₂₄NO₄	342.1697	342.1705	-2.3	297.11, 265.09	1.00
Lotusine(3)	8.6	C₁₉H₂₄NO₃	314.1749	314.1756	-2.2	269.12, 237.09	3.97
Menisperine(4)	10.6	C₂₁H₂₆NO₄	356.1863	356.1862	0.3	311.13, 279.10	5.16
Palmatine(5)	12.0	C₂₁H₂₂NO₄	352.1549	352.1549	0	336.09, 322.07, 308.09, 294.08	30.76
Columbamine(6)	13.0	C₂₀H₂₀NO₄	338.1389	338.1392	-0.9	322.11, 308.09, 294.11, 280.10	2.10
Jatrorrhizine(7)	13.2	C₂₀H₂₀NO₄	338.1390	338.1392	-0.6	322.11, 308.09, 294.11, 280.10	7.17
Berberrubine(8)	14.0	C₁₉H₁₆NO₄	322.1078	322.1079	-0.3	307.08	22.09
Berberine(9)	15.0	C₂₀H₁₈NO₄	336.1233	336.1236	-0.9	320.09, 306.08, 292.10, 278.08	15.02

Compound	t_R/min	Formula	Measured, m/z	Theory, m/z	10⁶ Error	MS², m/z	Binding degree(%)
Phellodendrine(1)	5.2	C₂₀H₂₄NO₄	342.1698	342.1705	-2.0	192.10	3.25
Magnoflorine(2)	6.2	C₂₀H₂₄NO₄	342.1697	342.1705	-2.3	297.11, 265.09	1.00
Lotusine(3)	8.6	C₁₉H₂₄NO₃	314.1749	314.1756	-2.2	269.12, 237.09	3.97
Menisperine(4)	10.6	C₂₁H₂₆NO₄	356.1863	356.1862	0.3	311.13, 279.10	5.16
Palmatine(5)	12.0	C₂₁H₂₂NO₄	352.1549	352.1549	0	336.09, 322.07, 308.09, 294.08	30.76
Columbamine(6)	13.0	C₂₀H₂₀NO₄	338.1389	338.1392	-0.9	322.11, 308.09, 294.11, 280.10	2.10
Jatrorrhizine(7)	13.2	C₂₀H₂₀NO₄	338.1390	338.1392	-0.6	322.11, 308.09, 294.11, 280.10	7.17
Berberrubine(8)	14.0	C₁₉H₁₆NO₄	322.1078	322.1079	-0.3	307.08	22.09
Berberine(9)	15.0	C₂₀H₁₈NO₄	336.1233	336.1236	-0.9	320.09, 306.08, 292.10, 278.08	15.02