Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (5): 856.doi:

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A Novel Method for Capillary Electrophoresis Directly Analyzing Trace Amounts Analytes in the Water-immiscible Solution Samples

FANG Huai-Fang, ZENG Zhao-Rui*, LIU Lan, PANG Dai-Wen   

  1. College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
  • Contact: ZENG Zhao-Rui

Abstract:

This paper describes a novel method in which solvent microextraction(SME) is followed by on\|line back-extraction-field amplified sample injection(OLBE-FASI) in capillary zone electrophoresis(CZE). The water-immiscible solution sample, sealed with water plug in the sample vial, was used for direct electroinjection. The water plug with moderate content of organic solvent, low-conductivity, and the presence of a small amount of H+ provided the highest sensitivity for analyzing positively chargeable compounds, such as cocaine and thebaine. The linear range was 0.040—4, 0.040—2 μg/mL, with the square of the correlation coefficient(r2)>0.997, respectively. Detection limits were in the range of 5—10 ng/mL.

Key words: Solvent microextraction; On-line back-extraction; Field amplified sample injection

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