Disturbance of Troxerutin to Belousov-Zhabotinskii Oscillating System and Its Content Determination†

doi:10.7503/cjcu20150481

Abstract

Abstract:

A new method for the determination of troxerutin was developed based on the disturbance of troxerutin to Belousov-Zhabotinskii oscillation system. Under optimal conditions, the variation of ΔE had a linear relationship with troxerutin concentration in the range of 0.808—40.4 μmol/L, with detection limit of 2.14×10^-7 mol/L(S/N=3). The established method was used to determination of troxerutin in injection, and the recovery was 98.8%—101%. The results showed that the data obtained by this method and the ones by UV method had no significant difference. The possible mechanism was studied using cyclic voltammetry and UV spectrophotometry.

Key words: Belousov-Zhabotinskii oscillation, Troxerutin, Chemical oscillation

CLC Number:

O652
O615.4

TrendMD:

YE Danni, CAI Zhuo, JIANG Cuiwen, YUE Weichao. Disturbance of Troxerutin to Belousov-Zhabotinskii Oscillating System and Its Content Determination^†[J]. Chem. J. Chinese Universities, 2016, 37(1): 31.

Figures/Tables 9

Fig.1 Schematic diagram of the experimental apparatus

Fig.2 Oscillation spectrum of B-Z oscillating system^System conditions: 0.75 mol/L H2SO4+100.0 mmol/L KBrO3+2.0 mmol/L Ce4+ +50.0 mmol/L CH2(COOH)2.

Fig.3 Oscillation spectra of B-Z oscillating system with 28.3 μmol/L(A) and 40.4 μmol/L troxerutin(B)

Fig.4 Effects of temperature(A), stirring rate(B) and concentrations of KBrO3(C), Ce4+(D), malonic acid(E) and H2SO4(F) on ΔE

Table 1 Maximum ratio of some interfering species

Species added	Maximum tolerable concentration ratio	Species added	Maximum tolerable concentration ratio
K⁺, Cl^-, Na⁺, N	1000	I^-, Br^-	50
S, S	800	Ethanol	20
Ca²⁺, Zn²⁺	500	Mn²⁺, Cu²⁺	10

Table 2 Results of determination of troxerutin in injection(n=5)*

Sample No.	Labeled amount/mg	Present method(mg)	Average±SD/mg	UV Method(mg)	Average±SD/mg
13072571	60	60.2	60.0±0.5	60.3	60.0±0.3
		60.4		60.7
		60.3		60.1
		59.5		60.1
		59.4		59.8
13072631	60	60.0	59.8±0.3	60.7	60.0±0.5
		59.3		59.7
		60.0		60.3
		60.0		59.5
		59.9		60.1

Table 3 Results of recovery(n=5)

c(Sample)/(μmol·L^-1)	c(Added)/(μmol·L^-1)	c(Found)/(μmol·L^-1)	Recovery(%)	RSD(%)
16.2	4.05	20.2	98.8	1.2
	8.09	24.4	101
	12.1	28.2	99.1
	16.2	32.2	98.8
	20.2	36.6	101
6.06	2.02	8.10	101	1.3
	6.06	12.0	98.0
	8.08	14.2	101
	10.1	16.1	99.4
	12.1	18.1	99.5

Fig.5 UV absorption spectra of different systems ^a. 2.59×10-5 mol/L troxerutin; b. 2.59×10-5 mol/L troxerutin+0.1 mol/L H2SO4; c. 2.59×10-5 mol/L troxerutin+4.0 mmol/L CH2(COOH)2; d. 2.59×10-5 mol/L troxerutin+2.0 mmol/L Ce4+; e. 2.59×10-5 mol/L troxerutin+5.0 mmol/L KBrO3.

Fig.6 Cyclic voltammograms of different systems ^ a. 0.5 mol/L H2SO4; b. 0.5 mol/L H2SO4+2.4 mmol/L troxerutin; c. 0.5 mol/L H2SO4+2.4 mmol/L troxerutin+1.0 mmol/L Ce4+.

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