Non-linear Chemical Analysis of Throng Component and Simultaneous Determining Contents of Cow and Mare Milks Mixed with Goat Milk†

doi:10.7503/cjcu20150049

Abstract

Abstract:

A method was proposed to determine the contents of raw milks in mixed milks. At first, non-linear chemical fingerprints of standard samples of goat milk and mixed milks with different contents of raw milks were determined at different temperatures, and via the multiple linear relationship between the contents of raw milks and the fingerprint-inductive-time difference of the mixed milks relative to goat milk, an analysis system model to predict the contents was fitted through classical least squares(CLS). And then, using the inductive-time difference between the sample and goat milk, the raw milk contents were calculated by the model. Lastly, according to the raw contents, the authenticity and quality of the sample were identified and evaluated. The results show that the correlation coefficient between the measured values and true values is 0.9991, the RSDs are 0.77%—4.3%, the recoveries are 93.2%—105.5%, and the detection limits of cow and mare milks doped in goat milk are 1.93% and 2.81%, respectively. The method has good reproducibility and accuracy, and is a practical and exact one for determining the raw milk contents in mixed milks, identifying the authenticity of dairy, and evaluating the quality of mixed milk. The method can be used for reference in research on determining throng component in other samples.

Key words: Non-linear chemical analysis, Throng component, Multiple milk mixture, Authenticity identifi-cation, Determination of content

CLC Number:

O655

TrendMD:

WANG Erdan, LU Lili, ZHANG Taiming, DONG Wenbin, LU Hongmei, LIANG Yizeng, XU Tianshu, ZHANG Juan, CHEN Chunnan. Non-linear Chemical Analysis of Throng Component and Simultaneous Determining Contents of Cow and Mare Milks Mixed with Goat Milk^†[J]. Chem. J. Chinese Universities, 2015, 36(6): 1052.

Figures/Tables 6

References 26

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Standard sample		45 ℃		50 ℃
Name	Constituent(%)	t_ind/s	y=Δt_ind/s	t_ind/s	y=Δt_ind/s
Goat milk	x₃ = 100	3842	0	2549	0
Mixed milk 1	x₁=18.0, x₂=2.00, x₃=80.0	3462	-380	2331	-218
Mixed milk 2	x₁=27.0, x₂=20.0, x₃=53.0	3128	-714	2164	-385
Mixed milk 3	x₁=41.0, x₂=30.0, x₃=29.0	2844	-998	2003	-546

Standard sample		45 ℃		50 ℃
Name	Constituent(%)	t_ind/s	y=Δt_ind/s	t_ind/s	y=Δt_ind/s
Goat milk	x₃ = 100	3842	0	2549	0
Mixed milk 1	x₁=18.0, x₂=2.00, x₃=80.0	3462	-380	2331	-218
Mixed milk 2	x₁=27.0, x₂=20.0, x₃=53.0	3128	-714	2164	-385
Mixed milk 3	x₁=41.0, x₂=30.0, x₃=29.0	2844	-998	2003	-546

Sample No.	y=-Δt_ind/s		x₁				x₂				x₃				Evaluation result^b
Sample No.	45 ℃	50 ℃	Measured value (%)	True value (%)		RSD (%)	Recovery (%)	Measured value (%)	True value (%)	RSD (%)	Recovery (%)	Measured value (%)	True value (%)	RSD (%)	Recovery (%)
1	1991	1154	97.33	100.0	1.2	97.33	1.45	0.000	3.5		1.22	0.000	3.9		Cow milk
2	25.7	15.8	1.48	0.000	3.7		-0.71	0.000	4.3		99.23	100.0	0.77	100.0	Goat milk
3	1822	1055	88.97	90.0	1.5	98.86	1.59	0.000	3.5		9.44	10.0	2.75	94.4	G-C milk
4	495	187	-1.03	0.000	4.0		82.11	80.0	1.6	102.6	19.52	20.0	1.9	97.6	G-M milk
5	585	337	28.11	28.00	1.7	100.4	1.91	2.00	3.2	95.7	69.98	70.00	1.1	99.97	G-C-M milk
6	849	469	35.82	35.00	1.6	102.3	18.96	20.00	1.8	94.8	45.22	45.00	1.5	100.5	G-C-M milk
7	1401	776	59.40	60.00	1.3	99.0	30.40	30.00	1.5	101.3	10.2	10.00	2.7	102	G-C-M milk
8	825	381	15.82	15.00	2.1	105.5	79.97	81.00	1.0	98.73	4.21	4.00	3.5	105.3	G-C-M milk
9	1096	627	51.38	52.00	1.4	98.8	7.93	8.00	2.9	99.1	40.69	40.00	1.4	101.7	G-C-M milk
10	1338	760	61.60	60.00	1.2	102.7	13.25	13.00	2.4	101.9	25.15	27.00	1.6	93.2	G-C-M milk

Sample No.	y=-Δt_ind/s		x₁				x₂				x₃				Evaluation result^b
Sample No.	45 ℃	50 ℃	Measured value (%)	True value (%)		RSD (%)	Recovery (%)	Measured value (%)	True value (%)	RSD (%)	Recovery (%)	Measured value (%)	True value (%)	RSD (%)	Recovery (%)
1	1991	1154	97.33	100.0	1.2	97.33	1.45	0.000	3.5		1.22	0.000	3.9		Cow milk
2	25.7	15.8	1.48	0.000	3.7		-0.71	0.000	4.3		99.23	100.0	0.77	100.0	Goat milk
3	1822	1055	88.97	90.0	1.5	98.86	1.59	0.000	3.5		9.44	10.0	2.75	94.4	G-C milk
4	495	187	-1.03	0.000	4.0		82.11	80.0	1.6	102.6	19.52	20.0	1.9	97.6	G-M milk
5	585	337	28.11	28.00	1.7	100.4	1.91	2.00	3.2	95.7	69.98	70.00	1.1	99.97	G-C-M milk
6	849	469	35.82	35.00	1.6	102.3	18.96	20.00	1.8	94.8	45.22	45.00	1.5	100.5	G-C-M milk
7	1401	776	59.40	60.00	1.3	99.0	30.40	30.00	1.5	101.3	10.2	10.00	2.7	102	G-C-M milk
8	825	381	15.82	15.00	2.1	105.5	79.97	81.00	1.0	98.73	4.21	4.00	3.5	105.3	G-C-M milk
9	1096	627	51.38	52.00	1.4	98.8	7.93	8.00	2.9	99.1	40.69	40.00	1.4	101.7	G-C-M milk
10	1338	760	61.60	60.00	1.2	102.7	13.25	13.00	2.4	101.9	25.15	27.00	1.6	93.2	G-C-M milk

Doped milk	t/℃	x₁(%)	x₂(%)	x₃(%)	R=-Δt_ind/s	S(s/100%)	S formula	N/s	D(%)	D formula
Cow milk	50	50	0	50	621	1242	S=R/x₁	8	1.93	D=3N/S
Mare milk	50	0	50	50	427	854	S=R/x₂	8	2.81	D=3N/S