Chem. J. Chinese Universities ›› 2018, Vol. 39 ›› Issue (7): 1440.doi: 10.7503/cjcu20170752
• Analytical Chemistry • Previous Articles Next Articles
TAN Xueying1, ZHANG Taiming1, DENG Feiyue1,*(), HUANG Jian1, WEN Qing2, CHEN Chunnan1
Received:
2017-11-20
Online:
2018-07-10
Published:
2018-06-21
Contact:
DENG Feiyue
E-mail:135261@csu.edu.cn
Supported by:
CLC Number:
TrendMD:
TAN Xueying, ZHANG Taiming, DENG Feiyue, HUANG Jian, WEN Qing, CHEN Chunnan. Identification of Different Acorus calamus and Determination of Content of Acorus calamus in Binary Mixtures by Nonlinear Chemical Fingerprint†[J]. Chem. J. Chinese Universities, 2018, 39(7): 1440.
Fig.2 Nonlinear chemical fingerprint of 0.5000 g Grass-leaved sweetflag and its part of feature information^a-b is inductive curve; b-c is undulatory curve; a and c are the beginning and the end points of nonlinear chemical reactions, respectively.
Fig.3 Reproducibility(A—C) and characteristic difference(D) of the fingerprints of Grass-leaved sweetflag(A), Rhizoma calami(B) and Acorus graminens soland(C)^(A)—(C) a—c represent three parallel measurements; (D) a. Acorus graminens soland; b. Grass-leaved sweetflag; c. Rhizoma calami.
Parameter | Eigenvalue | Parameter | Eigenvalue | ||||
---|---|---|---|---|---|---|---|
Grass-leaved sweetflag | Rhizoma calami | Acorus graminens soland | Grass-leaved sweetflag | Rhizoma calami | Acorus graminens soland | ||
tcan/s | 276.3 | 534.5 | 600.8 | τund/s | 49.34 | 51.22 | 54.90 |
Ecan/V | 0.4430 | 0.4990 | 0.4350 | tune/s | 9447 | 7741 | 11182 |
tpet/s | 1895 | 925.0 | 936.7 | Eune/V | 0.8700 | 0.8800 | 0.8640 |
Epet/V | 1.144 | 1.166 | 1.153 | tund/s | 4962 | 4340 | 6353 |
tind/s | 4485 | 3401 | 4829 | ΔEmax/V | 0.1550 | 0.1360 | 0.1340 |
Euns/V | 0.8860 | 0.9030 | 0.8980 | nwav | 108 | 99 | 117 |
Table 1 Eigenvalue of nonlinear chemical fingerprints parameters of three Acorus calamus(n=3)
Parameter | Eigenvalue | Parameter | Eigenvalue | ||||
---|---|---|---|---|---|---|---|
Grass-leaved sweetflag | Rhizoma calami | Acorus graminens soland | Grass-leaved sweetflag | Rhizoma calami | Acorus graminens soland | ||
tcan/s | 276.3 | 534.5 | 600.8 | τund/s | 49.34 | 51.22 | 54.90 |
Ecan/V | 0.4430 | 0.4990 | 0.4350 | tune/s | 9447 | 7741 | 11182 |
tpet/s | 1895 | 925.0 | 936.7 | Eune/V | 0.8700 | 0.8800 | 0.8640 |
Epet/V | 1.144 | 1.166 | 1.153 | tund/s | 4962 | 4340 | 6353 |
tind/s | 4485 | 3401 | 4829 | ΔEmax/V | 0.1550 | 0.1360 | 0.1340 |
Euns/V | 0.8860 | 0.9030 | 0.8980 | nwav | 108 | 99 | 117 |
Sample | System similarity | ||
---|---|---|---|
Mutual mode of Grass-leaved sweetflag | Mutual mode of Rhizoma calami | Mutual mode of Acorus graminens soland | |
Grass-leaved sweetflag | 0.9990 | 0.8857 | 0.8839 |
0.9993 | 0.8854 | 0.8837 | |
0.9992 | 0.8855 | 0.8841 | |
Rhizoma calami | 0.8841 | 0.9989 | 0.9123 |
0.8839 | 0.9993 | 0.9120 | |
0.8845 | 0.9990 | 0.9119 | |
Acorus graminens soland | 0.8823 | 0.9120 | 0.9992 |
0.8815 | 0.9115 | 0.9995 | |
0.8818 | 0.9910 | 0.9989 |
Table 2 System similarities calculated by taking common pattern characteristic parameters(n=3) as references
Sample | System similarity | ||
---|---|---|---|
Mutual mode of Grass-leaved sweetflag | Mutual mode of Rhizoma calami | Mutual mode of Acorus graminens soland | |
Grass-leaved sweetflag | 0.9990 | 0.8857 | 0.8839 |
0.9993 | 0.8854 | 0.8837 | |
0.9992 | 0.8855 | 0.8841 | |
Rhizoma calami | 0.8841 | 0.9989 | 0.9123 |
0.8839 | 0.9993 | 0.9120 | |
0.8845 | 0.9990 | 0.9119 | |
Acorus graminens soland | 0.8823 | 0.9120 | 0.9992 |
0.8815 | 0.9115 | 0.9995 | |
0.8818 | 0.9910 | 0.9989 |
Fig.4 Characteristic fingerprint of different batches of Grass-leaved sweetflag 18(A), Grass-leaved sweetflag 20(B), Grass-leaved sweetflag 22(C) and Grass-leaved sweetflag 24(D)^ a—c represent reproducibility of three parallel measurements.
xRhizoma calami(%) | tind 1/min | tune 1/min | xAcorus graminens soland(%) | tind 2/min | tune 2/min |
---|---|---|---|---|---|
0 | 74.70 | 155.1 | 0 | 74.70 | 155.0 |
10.0 | 72.93 | 151.0 | 10.0 | 75.08 | 158.1 |
30.0 | 69.65 | 145.3 | 30.0 | 76.00 | 162.1 |
50.0 | 65.73 | 140.1 | 50.0 | 77.11 | 167.3 |
70.0 | 61.83 | 135.0 | 70.0 | 78.30 | 173.0 |
90.0 | 58.45 | 130.2 | 90.0 | 79.35 | 178.5 |
100 | 56.65 | 127 | 100 | 79.60 | 182.00 |
Table 3 Influence of doping contents of mixtures consisting of two species of Acorus calamus on the inductive time(tind) and the undulation end time(tune)
xRhizoma calami(%) | tind 1/min | tune 1/min | xAcorus graminens soland(%) | tind 2/min | tune 2/min |
---|---|---|---|---|---|
0 | 74.70 | 155.1 | 0 | 74.70 | 155.0 |
10.0 | 72.93 | 151.0 | 10.0 | 75.08 | 158.1 |
30.0 | 69.65 | 145.3 | 30.0 | 76.00 | 162.1 |
50.0 | 65.73 | 140.1 | 50.0 | 77.11 | 167.3 |
70.0 | 61.83 | 135.0 | 70.0 | 78.30 | 173.0 |
90.0 | 58.45 | 130.2 | 90.0 | 79.35 | 178.5 |
100 | 56.65 | 127 | 100 | 79.60 | 182.00 |
Fig.7 Linear relationship between the inductive time(A) or undulation end time(B) of the mixture fingerprint and the content of Acorus graminens soland
No. | tind/min | Measured value(%) | Ture value(%) | AD (%) | RD (%) | RSD (%) | tune/min | Measured value(%) | Ture value(%) | AEa (%) | REb (%) | RSD (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 74.7 | 0.47 | 0.0 | 0.47 | — | 0.04 | 155.3 | -4.87 | 0.0 | -4.87 | — | 0.27 |
2 | 71.2 | 19.92 | 20.0 | 0.08 | 0.4 | 0.16 | 148.5 | 20.21 | 20.0 | 0.21 | 1.05 | 0.48 |
3 | 66.4 | 46.18 | 45.0 | 1.18 | 2.62 | 0.21 | 142.0 | 44.19 | 45.0 | -0.81 | 1.8 | 1.00 |
4 | 60.4 | 79.15 | 80.0 | 0.85 | 1.06 | 0.47 | 132.1 | 81.07 | 80.0 | 1.07 | 1.33 | 0.02 |
5 | 56.7 | 99.62 | 100 | 0.38 | 0.38 | 0.06 | 128.3 | 95.83 | 100 | -4.17 | 4.17 | 2.7 |
Table 4 Results and their accuracies to quantitatively analyze the mixtures consisting of Rhizoma calami and Grass-leaved sweetflag via the linear relationship between the doping content and the tind or tune
No. | tind/min | Measured value(%) | Ture value(%) | AD (%) | RD (%) | RSD (%) | tune/min | Measured value(%) | Ture value(%) | AEa (%) | REb (%) | RSD (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 74.7 | 0.47 | 0.0 | 0.47 | — | 0.04 | 155.3 | -4.87 | 0.0 | -4.87 | — | 0.27 |
2 | 71.2 | 19.92 | 20.0 | 0.08 | 0.4 | 0.16 | 148.5 | 20.21 | 20.0 | 0.21 | 1.05 | 0.48 |
3 | 66.4 | 46.18 | 45.0 | 1.18 | 2.62 | 0.21 | 142.0 | 44.19 | 45.0 | -0.81 | 1.8 | 1.00 |
4 | 60.4 | 79.15 | 80.0 | 0.85 | 1.06 | 0.47 | 132.1 | 81.07 | 80.0 | 1.07 | 1.33 | 0.02 |
5 | 56.7 | 99.62 | 100 | 0.38 | 0.38 | 0.06 | 128.3 | 95.83 | 100 | -4.17 | 4.17 | 2.7 |
No. | tind/min | Measured value(%) | Ture value(%) | AD (%) | RD (%) | RSD (%) | tune/min | Measured value(%) | Ture value(%) | AE (%) | RE (%) | RSD (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 74.7 | 2.93 | 0.0 | 2.93 | — | 0.04 | 155.0 | 1.32 | 0.0 | 1.32 | — | 0.14 |
2 | 75.6 | 19.74 | 20.0 | -0.26 | -1.3 | 0.52 | 159.5 | 20.14 | 20.0 | 0.14 | 0.70 | 0.44 |
3 | 76.9 | 44.03 | 45.0 | -0.97 | -2.16 | 0.63 | 166.7 | 45.37 | 45.0 | 0.37 | 0.82 | 0.85 |
4 | 78.7 | 77.65 | 80.0 | -2.35 | 2.93 | 0.76 | 175.5 | 78.51 | 80.0 | -1.49 | -1.87 | 0.81 |
5 | 80.0 | 101.94 | 100.0 | 1.94 | 1.94 | 0.22 | 181.2 | 99.22 | 100.0 | -0.78 | -0.78 | 0.39 |
Table 5 Results and their accuracies to quantitatively analyze the mixtures consisting of Acorus graminens soland and Grass-leaved sweetflag via the linear relationship between the doping content and the tind or tune
No. | tind/min | Measured value(%) | Ture value(%) | AD (%) | RD (%) | RSD (%) | tune/min | Measured value(%) | Ture value(%) | AE (%) | RE (%) | RSD (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 74.7 | 2.93 | 0.0 | 2.93 | — | 0.04 | 155.0 | 1.32 | 0.0 | 1.32 | — | 0.14 |
2 | 75.6 | 19.74 | 20.0 | -0.26 | -1.3 | 0.52 | 159.5 | 20.14 | 20.0 | 0.14 | 0.70 | 0.44 |
3 | 76.9 | 44.03 | 45.0 | -0.97 | -2.16 | 0.63 | 166.7 | 45.37 | 45.0 | 0.37 | 0.82 | 0.85 |
4 | 78.7 | 77.65 | 80.0 | -2.35 | 2.93 | 0.76 | 175.5 | 78.51 | 80.0 | -1.49 | -1.87 | 0.81 |
5 | 80.0 | 101.94 | 100.0 | 1.94 | 1.94 | 0.22 | 181.2 | 99.22 | 100.0 | -0.78 | -0.78 | 0.39 |
Temperature/℃ | xRhizoma calami(%) | xAcorus graminens soland(%) | xGrass-leaved sweetflag(%) | R/s | Sa(s/100%) | N/s | Db(%) |
---|---|---|---|---|---|---|---|
40 | 50 | — | 50 | 550.2 | 1100.4 | 4 | 1.09 |
40 | — | 50 | 50 | 144.6 | 289.2 | 4 | 4.15 |
Table 6 Sensitivities and detection limits to determine the contents of Rhizoma calami and Acorus graminens soland in the mixitures consisting of 2 species of Acorus calamus(n=5)
Temperature/℃ | xRhizoma calami(%) | xAcorus graminens soland(%) | xGrass-leaved sweetflag(%) | R/s | Sa(s/100%) | N/s | Db(%) |
---|---|---|---|---|---|---|---|
40 | 50 | — | 50 | 550.2 | 1100.4 | 4 | 1.09 |
40 | — | 50 | 50 | 144.6 | 289.2 | 4 | 4.15 |
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