Detection of Micro-metastatic Lymph Node in Thyroid Cancer by Fourier Transform Infrared Spectroscopy

doi:10.7503/cjcu20130528

Abstract

Abstract:

Sixteen non-and micro-metastatic freshly removed lymph nodes were measured by Fourier transform infrared spectrometor(FTIR) equipped with attenuated total reflectance(ATR) probe. For each spectrum, 38 variables of 13 bands including peak positions, relative intensities and full width at half maximum(FWHM) were compared between the two groups. The results indicated that the FTIR spectra of micro-metastatic lymph nodes were significantly different from non-metastatic in the bands related to protein, lipid, carbohydrate and nucleic acid. The relative intensity ratios of I₃₂₈₀/I₁₄₆₀, I₁₆₄₀/I₁₄₆₀ that related to protein increased significantly; the FWHM of F₁₆₄₀ increased, on the contrary, F₁₅₄₆ decreased significantly. The relative intensity ratios of I₁₄₀₀/I₁₄₆₀ that related to lipid increased significantly. The relative intensity ratio of I₁₁₆₅/I₁₄₆₀ that related to carbohydrate decreased significantly. The relative intensity ratio of I₁₂₄₀/I₁₄₆₀ that related to nucleic acid increased significantly. FTIR spectroscopy could be a potential powerful approach formicro-metastatic lymph nodes detection during the operation of thyroid cancer.

Key words: Thyroid cancer, Micro-metastatic, Lymph node, Fourier transform infrared spectroscopy

CLC Number:

O657.33

TrendMD:

LIU Ya-Qi, SANG Chang-Ye, XU Yi-Zhuang, XU Zhi, ZHOU Xiao-Si, LING Xiao-Feng. Detection of Micro-metastatic Lymph Node in Thyroid Cancer by Fourier Transform Infrared Spectroscopy[J]. Chem. J. Chinese Universities, 2013, 34(10): 2279.

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