Concentration Variation of Middle Molecules in Sera of Chronic Renal Failure Patients and Inhibition for Na,K-ATPase

Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (11): 2066.

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Concentration Variation of Middle Molecules in Sera of Chronic Renal Failure Patients and Inhibition for Na,K-ATPase

YANG Mei¹, CHU Jie-Gen¹, ZHANG Jian-Da¹, YUAN Zhi¹, YANG Hong-Tao², LIU Hong-Fu³, ZHAO Jing-Li², YANG Shu-Fen³

1. Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China;
2. First Teaching Hospital,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;
3. Tianhe Hospital,Tianjin 300050,China

Received:2004-10-10 Online:2005-11-10 Published:2005-11-10

Abstract

Abstract: Sera from patients with different courses of chronic renal failure and healthy persons were separated by gel permeation chromatography,and the concentration of middle molecular fraction B were calculated.The concentration of fractions B was increasing with the aggravating of renal failure degree,which indicated that the concentration of fraction B was related to patient's renal failure.Fractions B from uremic urine,uremic sera or normal urine were resolved into 10 to 17 sub-fractions by ion exchange chromatography.Sub-fractions from normal urine and from uremic sera were assayed for their inhibition for the activity of Na,K-ATPase.The results showed that the Na,K-ATPase activities of the samples with B-8 and B-9 were much lower than that of blank.It is indicated that B-8 and B-9 contained some compounds which could inhibit the activity of Na,K-ATPase.

Key words: Chronic renal failure, Middle molecule, Na,K-ATPase

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YANG Mei, CHU Jie-Gen, ZHANG Jian-Da, YUAN Zhi, YANG Hong-Tao, LIU Hong-Fu, ZHAO Jing-Li, YANG Shu-Fen. Concentration Variation of Middle Molecules in Sera of Chronic Renal Failure Patients and Inhibition for Na,K-ATPase[J]. Chem. J. Chinese Universities, 2005, 26(11): 2066.

[1]	YANG Mei, ZHANG Jian-Da, WANG Peng, LIU Xiao-Hang, YUAN Zhi, LIU Hong-Fu, YANG Hong-Tao, YANG Shu-Fen, ZHAO Jing-Li . Separation and Identification of the Uremic Middle Molecules [J]. Chem. J. Chinese Universities, 2005, 26(4): 677.
[2]	CHU Jie-Gen, YUAN Zhi, WU Qiang, HE Bing-Lin . Separation and Analyses of Uremic Middle Molecules by Chromatography and Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry [J]. Chem. J. Chinese Universities, 2001, 22(7): 1166.
[3]	Yuan Zhi, Zhang Ji, Zhao Fenzhi, Mi Huaifeng, He Binglin . Isolation and Preliminary Characterization of Properties of Middle Molecules from Sera of Uremic Patients [J]. Chem. J. Chinese Universities, 1989, 10(4): 401.

Concentration Variation of Middle Molecules in Sera of Chronic Renal Failure Patients and Inhibition for Na,K-ATPase

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