Abstract: In present paper, chromatographic behavior of mixed liquid crystals used as stationary phases in GC has been studied. Their dependency on the temperature, the stability of supercooled state were compared with those of the homo-liquid crystals. The phase constants, relative polarity of some liquid crystals and their mixtures were determined. In general, the resolving powers of mixed liquid crystals are better than that of corresponding homo-liquid crystals.On the packed and the glass capillary columns coated with a mixed liquid crystal, some natural essential oil and synthetic mixtures have been separated.