The Use of TTT Active Amide Method in Solid-phase Peptide Synthesis——The Synthesis of the Growth Hormone Releasing Peptide and Its Biological Activity

Chem. J. Chinese Universities ›› 1988, Vol. 9 ›› Issue (5): 461.

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The Use of TTT Active Amide Method in Solid-phase Peptide Synthesis——The Synthesis of the Growth Hormone Releasing Peptide and Its Biological Activity

Xue Chubiao¹, Li Chongxi¹, Xing Qiyi¹, Dong Minghui²

1. Departmene of Chemistry, Peking University, Beijinn;
2. Institute of Zoology, Academia Sinica, Beijing

Received:1986-12-17 Online:1988-05-24 Published:1988-05-24

Abstract

Abstract: The use of tetrahydrothiazole-2-thione (TTT) active amide method in solid-phase peptide synthesis has been studied through the synthesis of the growth hormone releasing peptide using chloromethylated copoly (styrene-2% divinylben-zene) resin as polymeric support. It was found that the coupling reaction was fast, the yield in each coupling step was nearly quantitative, and the purity of the final crude peptide was satisfactory. The biological activity of the synthetic peptide is also presented.

Key words: Tetrahydrothiazole-2-thione, Active amide, Solid phase peptide synthesis, Growth hormone releasing peptide

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Xue Chubiao, Li Chongxi, Xing Qiyi, Dong Minghui. The Use of TTT Active Amide Method in Solid-phase Peptide Synthesis——The Synthesis of the Growth Hormone Releasing Peptide and Its Biological Activity[J]. Chem. J. Chinese Universities, 1988, 9(5): 461.

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The Use of TTT Active Amide Method in Solid-phase Peptide Synthesis——The Synthesis of the Growth Hormone Releasing Peptide and Its Biological Activity

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