Abstract: The electrochemical behavior of horse heart Cytochrome Cwas investigated in the presence of 4,4' -Dithiodipyridine as an electron transfer promoter on the gold minigrid optical transparent thin-layer electrode (Au-OTTLE). The formal potential E0' and electron transfer number n of the redox reaction of Cytochrome Cwere measured as 50 mV(vs. Ag/AgCl) and 1 respectively by thin-layer spectroelectrochemistry on Au-OTTLE. These data were also compared with the cyclic voltammetric results observed on Au-OTTLE > Au-microdisk electrode, and Au-disk electrode.

Key words: Cytochrome C,4,4' -Dithiodipyridine, Thin-Layer spectroelectrochemistry