Adhesion and Proliferation of BMSCs on the Surface of PEGDA-based Hydrogel by Low-temperature Plasma

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (2): 402.

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Adhesion and Proliferation of BMSCs on the Surface of PEGDA-based Hydrogel by Low-temperature Plasma

TAN Guo-Xin^1*, WANG Ying-Jun², ZHANG Shu-Jiang³, CHENG Rong¹, NING Cheng-Yun², LIU Xu-Jian²

1. Department of Applied Chemistry, Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangdong 510006, China;
2. Institute of Biomaterial, College of Material Science and Engineering, South China University of Technology, Guangdong 510640, China;
3. The first Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China

Received:2010-03-29 Revised:2010-07-28 Online:2010-02-10 Published:2011-02-23
Contact: Guo-Xin TAN E-mail:tanguoxin@126.com
Supported by:
国家自然科学基金(批准号: 50872035)资助.

Abstract

Abstract: Argon plasma treatment was used to improve the biocompatibility of the surface of photo-polymerization Poly (ethylence glycol) diacrylate(PEGDA)-2-hydroxyethyl methacrylate (HEMA) hydrogel. The adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) on the surface of the films was investigated in this paper. Surface structures of the PEGDA-based films before and after treated by Ar plasma were characterized by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. Results confirm that the surface energy of PEGDA/HEMA hydrogels increased from 45.9 to 70.3 mJ?m-2 after plasma treatment. Compared with the untreated sample, BMSCs spread out more filopodia after 3d incubation on treated films, and it entered cell division phase more quickly. BMSCs culture studies showed that cells grew better on the plasma treated surface, which was beneficial to its cell compatibility.

Key words: PEGDA-HEMA, Hydrogels, Plasma treatment, Adhesion and proliferation

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TAN Guo-Xin*, WANG Ying-Jun, ZHANG Shu-Jiang, CHENG Rong, NING Cheng-Yun, LIU Xu-Jian. Adhesion and Proliferation of BMSCs on the Surface of PEGDA-based Hydrogel by Low-temperature Plasma[J]. Chem. J. Chinese Universities, 2011, 32(2): 402.

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Adhesion and Proliferation of BMSCs on the Surface of PEGDA-based Hydrogel by Low-temperature Plasma

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