Abstract: As a pharmaceutical intermediate, L-serine plays an important role in organic synthesis and peptide synthesis. Also N-acetyl-D,L-3-methoxy-alanine is an intermediate in synthesis of L-serine from methyl α-bromo-β-methoxypropionate. In this thesis, a new method of resolution of N-acetyl-D,L-3-methoxy-alanine by immobilized cells with aminoacylase was studied. Several factors of this enzymatic reaction were investigated. The optimal temperature and pH are 50 ℃ and 7.0. In 10 mL reaction solution, the optimal concentration of N-acetyl-D,L-3-methoxy-alanine is 500 mmol/L and the amount of immobilized cells is 1 g. 10^-4 mol/L of Co²⁺ and Mg²⁺ are benefit to aminoacylase, while Cu²⁺ and Zn²⁺ show clearly inhibitory effect. Under the optimal conditions, molar conversion rate of N-acetyl-L-3-methoxy-alanine is 96%.

Key words: Aminoacylase, D,L-3-Methoxy-alanine, Immobilized cell, Chiral resolution