Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (6): 1143.

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Differential Proteomic Study of Human T Lymphocytic Leukemia Protein Induced by Ionizing Radiation

LIANG Feng1, SHI Ying2, ZHANG Xuan3, LIU Zhong-Ying1, LIU Zhi-Qiang2*   

  1. 1. School of PharmaceuticalSciences, Jilin University, Changchun 130021, China;
    2. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    3. School of Public Health, Jilin University, Changchun 130021, China
  • Received:2009-11-02 Online:2010-06-10 Published:2010-06-10
  • Contact: LIU Zhi-Qiang. E-mail: liuzq@ciac.jl.cn
  • Supported by:

    国家自然科学基金(批准号: 20675079, 20873137, 30672600)资助.

Abstract:

Two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry(MALDI-TOF-MS) have been acknowledged as the efficient methods in proteomics studies. In this report, the total proteins of human T lymphocytic leukemia(Jurkat cells) treated by ionizing radiation were separated with two-dimensional electrophoresis. The differential expression of cellular proteins induced by ionizing radiation was analyzed by PDQUEST software. Six differential protein spots were identified by MALDI-TOF-MS, including Lamin B1, heterogeneous nuclear ribonucleoprotein, p47 protein isoform a, alternative splicing factor 1, prohibitin, and 6-phosphogluconolactonase. The results may help researchers better understand the underlying mechanism involved in cellular protein alteration induced by ionizing radiation.

Key words: Proteome; Two-dimensional electrophoresis; Matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry(MALDI-TOF-MS); Jurkat cell

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