Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (9): 1767.

• Articles • Previous Articles     Next Articles

Analysis of Differential Protein Expression Patterns on Interaction Between Arabidopsis thaliana and Spodoptera exigua

LIU Lin-Lin1, XI Jing-Hui1*, LIAN Jie1, TANG Xin-Long1, AN Shao-Li1, SUN Li-Wen3, ZHANG Ju-Hong1, XU Peng1, PAN Yi-Ou1, LI Shan-Yu2*   

  1. 1. College of Plant Science, Jilin University, Changchun 130062, China;
    2. The First Clinical Hospital,
    3. Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, Changchun 130021, China
  • Received:2009-01-16 Online:2009-09-10 Published:2009-09-10
  • Contact: XI Jing-Hui. E-mail: jhxi1965@jlu.edu.cn; LI Shan-Yu. E-mail: shanyul@sina.com
  • Supported by:

    吉林省科技厅项目(批准号: 20060545)和长春市科技局国际合作项目(批准号: 2007GH28)资助.

Abstract:

A differential analysis of expression proteins on the interaction between Spodoptera exigua(genera-list) and Arabidopsis thaliana was studied via 2-DE combined with high-throughput MS. On the basis of the abundance of expression proteins in the model plant Arabidopsis fed by the insect, the intensities of 28 spots in more than 1100 protein spots detected on each 2D gel were significantly changed. Among them, 17 protein spots were up-regulated, and 11 were down-regulated under the insect feeding for 8 h. In addition, six responsive proteins of them were confirmed by MALDI-TOF MS analysis and in turn, the homogeneous comparison of protein sequences. These proteins were generally found in organism metabolism, such as transketolase, S-adenosylmethionine synthase, dihydrolipoamide dehydrogenase 2, β-ureidopropionase, and fatty-acid synthase, etc. Although the proteome for the interaction between the insect and Arabidopsis is complex enough, the important thing for us is how to elucidate the inducible defense mechanism of plants. Therefore, the above data may provide a new insight to the biotic stress response.

Key words: Spodoptera exigua; Arabidopsis thaliana; Two-dimensional electrophoresis; MALDI-TOF MS; Induced-defense

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