CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (8): 1553.

• Articles • Previous Articles     Next Articles

Research on Characterization and Biocompatibility of Nano-bacterial Cellulose Membrane

WANG Zong-Liang1,2, JIA Yuan-Yuan3, SHI Yi1, CONG Deng-Li1, CHEN Yan-Yan1, JIA Shi-Ru4, ZHOU Yu-Lai1*   

  1. 1. College of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
    2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    3. College of Material Science and Chemical Engineering,
    4. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Science and Technology University, Tianjin 300457, China
  • Received:2008-12-05 Online:2009-08-10 Published:2009-08-10
  • Contact: ZHOU Yu-Lai. E-mail: zhouyl@jlu.edu.cn
  • Supported by:

    吉林省科技厅项目(批准号: 20050401-2, 200705386)和天津科技大学人才引进基金(批准号: 20070443)资助.

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Abstract:

Bacterial cellulose membrane, possessing nanofibers, an ultrafine 3-dimensional network and proper porosity, was prepared by static culture of Acetobacter xylinum and characterized by light microscope, SEM(Scanning electron microscope) and AFM(Atomic force microscope). The results show that BC has extremely fine network of nano-structure, the pore size of freeze-dried BC is 0.6 to 2.8 μm; the width of cellulose is 50 to 80 nm. By measuring their dry weight and wet weight, the porosities of air-dried BC and freeze-dried BC were determined, respectively. The porosity of air-dried and freeze-dried BC is about 70% and 90%, respectively. The water vapor permeability of air-dried BC is quite excellent because the existence of a large number of hydroxyl. BC co-culture with fibroblasts(FBs) and chondrocytes, respectively, and subcutaneous implant of FBs-BC composition into nude mouse. The composition is well intergrated into the skin of nude mouse. FBs and chondrocytes form continuous cell layer on the surface of BC and the expression of GFP is normally. The results demonstrate that BC membrane is suitable for cell attachment and proliferation, and shows better biocompatibility. It is expected to become a promising tissue engineering scaffold.

Key words: Bacterial cellulose; Biocompatibility; Tissue engineering scaffold

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