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Chem. J. Chinese Universities

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Preparation of Velvet Antlers Small Peptides and Stimulating Effects on Osteosarcoma Cell Proliferation

WANG Hua1,2, LIN Zhe3, LIU Qiang2, CAI MING-Jun2, XU Li1*, ZHANG Xue-Zhong1*   

    1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China;
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, China;
    3. Research and Exploitation Center of Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China
  • Received:2008-02-29 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10
  • Contact: ZHANG Xue-Zhong, XU Li

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Abstract: The native total peptides(VATP)were extracted from velvet antlers of sika deer(Cerv us nippon Temminck). A high active peptide component(VAP-B)stimulating proliferation of a human osteosarcoma cells was isolated from VATP. Finally, a further purified active peptide component(VAP-B2) with molecular weight distribution from about 200—600 was obtained from VAP-B by preparative HPLC. VAP-B2 was able to enhance the average content of DNA in S phase. And also the alkali phosphatase(ALP) activity in osteosarcoma cells increased obviously with the increase of VAP-B2 dosage and treatment time. The results are consistent with cell cycle variation and ALP level as a marker of bond formation during osteocyte proliferation and differentiation, as well as maturity. The results obtained in this study provide a clue to explore a new peptide medicine from velvet antlers for therapy of osteoporosis.

Key words: Velvet antlers of sika deer, Sika deer peptides, Isolation and purification, Physiologic activity, Osteosarcoma cells

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