Preliminary Study on Hippocampal Proteomics of Senescence-accelerated Mouse

Chem. J. Chinese Universities

• 研究论文 • Previous Articles Next Articles

Preliminary Study on Hippocampal Proteomics of Senescence-accelerated Mouse

DONG Lei¹, JIANG Ning¹, ZHOU Wen-Xia¹, ZHANG Yong-Xiang1, GENG Miao¹, ZHANG Xue-Min², LIU Bing-Yu², WANG Jie²

1. Beijing Institute of Pharmacology and Toxicology,
2. National Center of Biomedical Analysis, Beijing 100850, China

Received:2006-02-27 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10
Contact: ZHOU Wen-Xia

Abstract

Abstract: To investigate mechanisms of the deficits of learning and memory related with aging, the differentially expressed hippocampal proteins from 6- and 12-month-old SAM-prone/8(SAMP8)and age-matched SAM-resistance/1(SAMR1)were analyzed and compared. In comparison with the same age SAMR1, 15 proteins expressions in hippocampus of 6-month-old SAMP8 increased, 5 proteins expressions decreased significantly; 12 proteins expressions in hippocampus of 12-month-old SAMP8 increased, 2 proteins expressions decreased significantly and 2 proteins only expressed in SAMP8 hippocampus; and 22 proteins with significant changes were identified by MALDI-TOF-MS and the results were searched in MASCOT database. These identified proteins could be devided into four categories according to their functions: (1) energy metabolism; (2) mitochondrion function; (3) signal transduction; (4) other proteins. The results show that there were significant differences in hippocampus protein expressions between SAMP8 and SAMR1, and some differentially expressed proteins were correlated with the deficits of learning and memory in SAMP8 with aging, and these proteins could provide clues for the study and discovery of new protein targets for improving intelligence drugs.

Key words: Senescence-accelerated mouse, Hippocampus, Proteomics

CLC Number:

TrendMD:

DONG Lei¹, JIANG Ning¹, ZHOU Wen-Xia¹, ZHANG Yong-Xiang1, GENG Miao¹, ZHANG Xue-Min², LIU Bing-Yu², WANG Jie². Preliminary Study on Hippocampal Proteomics of Senescence-accelerated Mouse[J]. Chem. J. Chinese Universities, doi: .

[1]	ZHAO Huanhuan, LI Cunyu, JIN Hong. Evaluation of Quantitative Phosphoproteomic Performance of Sequential Enrichment of Metal Oxide Affinity Chromatography for Phosphopeptides Using TMT 10-plex Isobaric Labeling [J]. Chem. J. Chinese Universities, 2021, 42(12): 3624.
[2]	LI Ying, YAO Jun, YANG Pengyuan, FAN Huizhi. Global Survey of Mouse Liver Protein Expression Using Liquid Isoelectric Focusing Prefractionation of Tryptic Peptides and LC-MS/MS^† [J]. Chem. J. Chinese Universities, 2015, 36(6): 1074.
[3]	PI Zifeng, WANG Qianqian, ZHANG Jing, Song Fengrui, LIU Zhiqiang. Effect of Schisandra Fruit on Neurochemicals in Hippocampus of Diabetic Encephalopathy Rat Using Online MD-HPLC-MS/MS^† [J]. Chem. J. Chinese Universities, 2015, 36(3): 442.
[4]	ZHENG Jun-Fang, HUA Lin, ZHANG Qiu-Xia, LI De-Long, HE Jun-Qi*. Proteomic Analysis of Olfactory Memory Related Proteins in Mice [J]. Chem. J. Chinese Universities, 2010, 31(8): 1535.
[5]	ZHENG Jun-Fang, LIU Hua, XIONG Ying, WANG Xiao-Zhu, HE Jun-Qi*. Proteomic Analysis of Fear Memory Related Proteins in Mice [J]. Chem. J. Chinese Universities, 2010, 31(4): 736.
[6]	FANG Cai-Wang, HUANG Qing-Yu, LING Xue-Ping, KE Cai-Huan, HUANG He-Qing. Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt [J]. Chem. J. Chinese Universities, 2010, 31(3): 507.
[7]	ZHAO Hui-Hui, YANG Fan, WANG Wei, WANG Hong-Xia, WEI Kai-Hua. Label Free Proteome Analysis of Plasma from Unstable Angina with Blood Stasis Syndrome Patients [J]. Chem. J. Chinese Universities, 2010, 31(2): 285.
[8]	SUN Zhi-Ling*, GAO Jue-Min, XU Zhi-Yang, WANG Zheng, WANG Fu-Qiang. Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy [J]. Chem. J. Chinese Universities, 2010, 31(10): 1970.
[9]	LIU Ming-Qi, ZHANG Yang, CHEN Yao-Han, YANG Peng-Yuan*. Novel Strategy for Glyco-peptide Database and High-throughput Searching [J]. Chem. J. Chinese Universities, 2010, 31(10): 1916.
[10]	LIU Chi-Bo, LIANG Yong*, PAN Chun-Qin, WANG Hai-Bao, MOU Si-Hua. Proteome Study of Differential Protein Expression in HBV-related Primary Hepatic Carcinoma [J]. Chem. J. Chinese Universities, 2009, 30(9): 1763.
[11]	HUANG Lin^1,2, CHEN Dong-Shi¹, YAN Li¹, FANG Cai-Wang^1,3, HUANG He-Qing^1,2,3. Differential Proteins of Sub-buccal Ganglions Selected and Identified with Proteomic Techniques in Aplysia* Under the Stress Condition of Cadmium Chloride [J]. Chem. J. Chinese Universities, 2009, 30(2): 314.
[12]	HUANG Fu-Sheng, CHEN Hai-Bin, HUANG Lin, HUANG He-Qing. Comparison Analysis of Proteome of Both Cerebral and Optic Ganalions in Sepia esculenta* [J]. Chem. J. Chinese Universities, 2009, 30(10): 1980.
[13]	LI Ying, WANG Yi*, QU Hai-Bin, CHENG Yi-Yu. Proteome Study of Differential Protein Expression in Acute Infracted Rat Heart after Verapamil Treatment [J]. Chem. J. Chinese Universities, 2008, 29(3): 515.
[14]	XU Kun, YANG Xiao-Da*, WANG Kui. La3+ Induced Binding of Calmodulin(CaM) to CaM-binding Proteins in Rat Brain Homogenate [J]. Chem. J. Chinese Universities, 2008, 29(12): 2525.
[15]	ZHAO Si-Hai^1,2, XUN Meng¹, CHU Yong-Lie^1*, ZHU Tong³, XUE Xin¹, XU Kun¹, SONG Juan¹, SHAO Ming-Ming¹. Comparative Proteomics Analysis of Huh7 Cells Harboring Intact HCV [J]. Chem. J. Chinese Universities, 2008, 29(11): 2174.

Preliminary Study on Hippocampal Proteomics of Senescence-accelerated Mouse

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments