Binary Chiral Selectors for Capillary Electrophoretic Enantioseparation of Amino Acids Derivatized with Fluoresceine Isothiocyanate

Abstract

Abstract: A novel capillary electrophoretic approach has been developed for the enantioseparation of aminio acids derivatized with fluoresceine isothiocyanate(FITC).The detection was performed with laser-induced fluorescence.Binary chiral selectors of -cyclodextrin( -CD) and sodium taurocholate(STC) were used and it was found that the resolution and enantioselectivity are superior to either -CDor STCused alone.The optimum molar ratio of -CDto STCis 23 at a total concentration above 20 mmol/L.The best running .buffer tried is 80 mmol/Lborate at pH = 9.3.In this work, all of the 20 pairs of amino acid enantiomers tested were baseline resolved with a resolution greater than 1.9 of which 17 pairs gave a resolution above than 3.0.The highest resolution reached 14.58.

Key words: Chiral separation, Amino acids, β-CD, STC, CE-LIF

CLC Number:

O657

TrendMD:

LU Xiao-Ning, CHEN Yi. Binary Chiral Selectors for Capillary Electrophoretic Enantioseparation of Amino Acids Derivatized with Fluoresceine Isothiocyanate[J]. Chem. J. Chinese Universities, 2002, 23(5): 822.

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Binary Chiral Selectors for Capillary Electrophoretic Enantioseparation of Amino Acids Derivatized with Fluoresceine Isothiocyanate

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