Abstract: This paper reports the electrochemical behaviour of hemoglobin at bare silver electrode.In the potential range of 0.4～-0.2 V(vs.SCE),a pair of redox peaks was observedat +0.26 V(oxidation) and +0.01 V(reduction)respectively at the sweep rate of 20 mV/swith an apparent electron-transfer rate constant(K_s)0.032 s^-1and electron-transfer number(n)0.94.The redox reaction showed such a good stability that the currents changed lessthan_0.2 μAunder a continued scan for 30 min,Hemoglobin was adsorped on the electrodesurface,but it was a faint adsorption.The electrode surface was not be stained during theredox reaction,In the medium of 0.1 mol/L NaAc-HAc(pH=4.5),both oxided current andreduced current are linear relationship with the concentration of hemoglobin in the ranges of2×10^-7～2×10^-6mol/Land 2×100^-5～1.5×10^-5mol/L.It can be used as a method for de-termining hemoglobin.

Key words: Hemoglobin, Direct electrocliemistry, Bare silver electrode