Abstract: The thermokinetie equation in the enzyme-catalytic reaction of the single substrate is suggested from the integral of the rate equation and the relationship between the variation of energy and the amount of reactants in the reaction and is tested with the hydrolysis reaction between arginine and arginase. The calculated values of the Michaelis constant K_m and the maximum rate V_max are 4.98×10^-3mol/Land 2.33×10^-5 mol/L·s, respectively, in agreement with those in the literature.

Key words: Thermokinetic equation, Michaelis constant, Microcalorimetric method