Chemical Enzymatic Analysis of Temporal Alterations in Mitochondrial Protein O - GlcNAc Modification during the Therapeutic Senescence Process of Breast Cancer Cells

doi:10.7503/cjcu20250121

Chem. J. Chinese Universities

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Chemical Enzymatic Analysis of Temporal Alterations in Mitochondrial Protein O - GlcNAc Modification during the Therapeutic Senescence Process of Breast Cancer Cells

JIANG Xinya^1#, MA Qile^2#, CHENG Hongying³, LIU Yin³, HUANG Huang, ZHAO Ran, LIU Yubo², ZHONG Xiaomin^1,3

1. Department of Oncology, The First Affiliated Hospital of Huai’an, Nanjing Medical University
2. Dalian University of Technology, Department of Life Sciences and Pharmacy
3. Department of Oncology, Huai’an Clinical College Affiliated to Xuzhou Medical University 4. Postdoctoral workstation of Dalian Hissen Bio Pharm, Inc

Received:2025-04-23 Revised:2025-05-22 Online First:2025-05-23 Published:2025-05-23
Contact: Yubo Liu E-mail:liuyubo@dlut.edu.cn
Supported by:

Supported by the Science and Technology Development Fund of the Affiliated Hospital of Xuzhou Medical University (No.XYFZ202204) and the Jiangsu Postgraduate Practice Innovation Program (No.SJCX24_1559)

1. ESM to 20250121.pdf(1035KB)

Abstract

Abstract: Cellular premature senescence is closely related to mitochondrial dysfunction. In this study, doxorubicin was used to induce breast cancer cells MCF-7 to establish a model of therapeutic premature senescence cells. The chemical enzymatic method based on GalT1 enzyme (Y289L) was employed to label the mitochondrial O-GlcNAc glycoproteome of MCF-7 cells at multiple time points during the premature senescence process. Click chemistry reaction was utilized to orthogonally capture mitochondrial glycoproteins. Label-free quantitative proteomics analysis was carried out through LC-MS/MS to investigate the temporal quantitative changes of mitochondrial O-GlcNAc glycoproteins during the cellular premature senescence process. Moreover, the biological function enrichment analysis was conducted to identify mitochondrial O-GlcNAc modified proteins involved in the regulation of premature senescence of breast cancer cells, and to elucidate the mechanism by which mitochondrial O-GlcNAc glycosylation regulates cellular premature senescence.

Key words: Chemoenzymatic labelling methods, Cell senescence, O-GlcNAcylation, Proteomics

CLC Number:

O657.6
Q51

TrendMD:

JIANG Xinya, MA Qile, CHENG Hongying, LIU Yin, HUANG Huang, ZHAO Ran, LIU Yubo, ZHONG Xiaomin. Chemical Enzymatic Analysis of Temporal Alterations in Mitochondrial Protein O - GlcNAc Modification during the Therapeutic Senescence Process of Breast Cancer Cells[J]. Chem. J. Chinese Universities, doi: 10.7503/cjcu20250121.

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Chemical Enzymatic Analysis of Temporal Alterations in Mitochondrial Protein O - GlcNAc Modification during the Therapeutic Senescence Process of Breast Cancer Cells

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