Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion†

doi:10.7503/cjcu20170160

Abstract

Abstract:

We constructed a fluorescent sensor for the detection of dichromate anion(Cr₂O^2-₇) on the basis of the quenching effect of Cr₂O^2-₇ on the fluorescence of cytidine-protected copper nanoclusters(CuNCs). The experimental results showed that the Cr₂O^2-₇ sensing system provided a linear range of 0.05—7.0 μmol/L with a low detection limit(S/N=3) of 24 nmol/L. In addition, the sensing system exhibited a good selectivity for Cr₂O^2-₇ detection, and could be applied for Cr₂O^2-₇ detection in lake water samples.

Key words: Copper nanoclusters, Dichromate, Fluorescence sensor

CLC Number:

O657

TrendMD:

CHEN Tianxia, ZHANG Zhengtao, WANG Yong, NI Yongnian. Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion^†[J]. Chem. J. Chinese Universities, 2017, 38(10): 1737.

Figures/Tables 6

Scheme 1 CuNCs-based fluorescent detection of Cr2O72-

Fig.1 TEM image(A), FTIR spectrum(B), photostability curve(C) of CuNCs and fluorescence intensity(D) of CuNCs vs. NaCl concentration

Fig.2 UV-Vis absorption spectrum of CuNCs(A) and fluorescence excitation(Ex) and emission(Em) spectra of CuNCs with and without 5.0 μmol/L Cr2O72- and UV-Vis absorption spectra of 5.0 μmol/L Cr2O72-(B)Insets of (B) are the optical images under 365 nm UV light.

Fig.3 Fluorescence emission spectra of CuNCs in the presence of different concentrations of Cr2O72-(A) and the relationship between ΔF and the concentration of Cr2O72- (B)(A) Concentration of CuNCs from top to bottom/(μmol·L-1): 0, 0.01, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 8.0, 10.0, 12.0, 14.0, 16.0, 18.0. Inset of (B) shows the corresponding linear calibration plot.

Fig.4 Fluorescence emission spectra of CuNCs in the presence or absence(blank) of each ion(A) and selectivity of CuNCs for Cr2O72-(B)(A) Concentration of Cr2O72- or any other ion is 1.0 μmol/L. Other ions include Blank, S2-, SCN-, S2O32-, Cl-, NO2-, F-, Br-, ClO3-, IO4-, BrO3-, PO43-, SO32-, H2PO4-, I-, Al3+, Pb3+, Fe3+, Mg2+, Cd2+, Cr3+, Co2+, Ba2+, Mn2+, Ca2+, Fe2+, Ni2+, Hg2+, Zn2+, Cu2+, NH4+, C15H34N+, C8H20N+. (B) ΔF=F0-F; F0 and F denote the fluorescence intensity without(blank) and with each ion, respectively. Ions from left to right: Cr2O72-, blank, S2-, SCN-, S2O32-, Cl-, NO2-, F-, Br-, ClO3-, IO4-, BrO3-, PO43-, SO32-, H2PO4-, I-, Al3+, Pb3+, Fe3+, Mg2+, Cd2+, Cr3+, Co2+, Ba2+, Mn2+, Ca2+, Fe2+, Ni2+, Hg2+, Zn2+, Cu2+, NH4+, C8H20N+, C15H34N+.

Table 1 Determination of Cr2O72- in spiked water samples(n=3)

Sample source	No.	Added/(μmol·L^-1)	Mean found/(μmol·L^-1)	Mean Recovery^*(%)	RSD(%)
Qingshan lake	1	0.50	0.59	118	8.3
	2	1.00	0.96	96	5.6
	3	1.50	1.48	99	2.9
Ganjiang river	1	0.50	0.49	98	3.5
	2	1.00	0.96	96	2.6
	3	1.50	1.62	108	3.9

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