Chemical Journal of Chinese Universities ›› 2013, Vol. 34 ›› Issue (1): 50-54.doi: 10.7503/cjcu20120185

• Article: Inorganic Chemistry • Previous Articles     Next Articles

Synthesis and Biocompatibility of Amphiphilic Graphene Oxide

LI Ning1,2,3, ZHANG Qi2, ZHANG Qing-Qing2,3, SONG Qin2, QI Lin2, WANG Long2,3, TANG Ming-Liang2, JIN Gang1,3, CHENG Guo-Sheng2,3   

  1. 1. Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China;
    2. Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-03-05 Published:2012-12-31


Amphiphilic functionalization of graphene oxide(GO) is of the essence for its biomedical applications. TWEEN 80-grafted GO was synthesized by a two-step approach. In step 1, GO surface was activated with hexamethylene diisocyanate(HMDI) through a reaction between GO carboxyl or hydroxyl groups and isocyanate. HMDI-activated GO was then grafted by TWEEN 80 in step 2, resulting in amphiphilic GO nano-sheets. The fabricated TWEEN 80-grafted GO exhibited excellent dispersibility in various solvents, such as water, chloroform and n-hexane. Furthermore, PC12 and HeLa cells were cultured on TWEEN 80-grafted GO film to assess its cytotoxicity. Cell morphology analysis and cell viability assay(Calcein AM &EthD-1 staining and MTT assay) indicated that the as-synthesized amphiphilic GO had good biocompatibility.

Key words: Amphiphilic graphene oxide, Biocompatibility, TWEEN 80, Hexamethylene diisocyanate

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