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Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (02): 389.doi: 10.3969/j.issn.0251-0790.2012.02.031

• Polymer Chemistry • Previous Articles     Next Articles

Preparation and Characterization of Gelatin/PLLA Composite Fibrous Scaffold and Evaluation for Corneal Cells Supports

YAN Jing1, GAO Yan1, WANG Qing2, CUI Xue-Jun1, ZHONG Shuang-Ling3, WANG Ping4, WANG Hong-Yan1   

  1. 1. College of Chemistry, Jilin University, Changchun 130012, China;
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, China;
    3. College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China;
    4. First Hospital, Jilin University, Changchun 130021, China
  • Received:2011-05-16 Online:2012-02-10 Published:2012-01-13

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Abstract: Gelatin and polylactic acid(PLLA) composite membranes were obtained via electrospun. The membranes composition on the surface properties, pore structure and mechanical properties were studied. The membranes were further used as scaffolds for rabbit corneal epithelial cells culture in vitro. The adhesion and proliferation of cells on these scaffolds were evaluated by immunofluorescence staining and thiazolyl bule tetrazolium bromide(MTT) assay. The results show that the fiber diameter distribution and surface hydrophilicity are significantly affected by composition of electrospinning solution. All of these scaffolds have high porosity and inter-connected pores. Gelatin could maintain high cell adhesion while PLLA improved mechanical properties of the composited scaffolds.

Key words: Gelatin, Poly-L-lactic acid, Composite scaffold, Corneal cell

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