高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

混合溶剂作淋洗剂的体积排除色谱(SEC)中溶剂化高分子峰和溶剂峰的关系

王庆国1, 李小文1, 刘波1, 蔡力行1, 程镕时2,3   

    1. 深圳大学化学与化工学院, 深圳 518060;
    2. 南京大学化学与化工学院, 南京 210093;
    3. 华南理工大学高分子研究所, 广州 510640
  • 收稿日期:2007-04-28 修回日期:1900-01-01 出版日期:2008-03-10 发布日期:2008-03-10
  • 通讯作者: 王庆国

Relationship Between Solvated Polymer Peak and Solvent Peak Using Mixed Solvent as Eluents in Size-exclusion Chromatography

WANG Qing-Guo1*, LI Xiao-Wen1, LIU Bo1, CAI Li-Xing1 , CHENG Rong-Shi2,3   

    1. College of Chemistry and Technology, Shenzhen University, Shenzhen 518060, China;
    2. College of Chemistry and Technology, Nanjing University, Nanjing 210008, China;
    3. Research Institute of Polymer Science, South China University of Technology, Guangzhou 510640, China
  • Received:2007-04-28 Revised:1900-01-01 Online:2008-03-10 Published:2008-03-10
  • Contact: WANG Qing-Guo

PDF (PC)

摘要： 以混合溶剂作淋洗剂的体积排除色谱(SEC)中高分子样品一般会出现2个峰, 分别是溶剂化高分子峰和自由溶剂峰. 理论分析结果表明, 溶剂化高分子峰面积( A3eff )是“裸高分子”峰面积(A3)和被束缚溶剂峰面积(A1*)的加和, 被束缚溶剂峰面积(A1*)和自由溶剂峰面积(A2*)大小相等符号相反. 以聚苯乙烯(3)-氯仿(1)-甲醇(2)体系为研究对象, 分别对A3eff, A3及A2*进行了实验测定, 证实了理论推断的正确性, 表明由高分子峰和由溶剂峰来求算优先吸附系数是等价的.

关键词: 体积排除色谱, 优先溶剂化, 混合溶剂

Abstract: In Size-exclusion Chromatography(SEC) two peaks are often observed for polymer samples when solvent mixtures are employed as eluents. These two peaks are solvated polymer peak and free solvent peak respectively. Theoretical analysis shows that the peak area of the solvated polymer A3eff is actually the sum of the “naked polymer” peak area A3 and the bound solvent peak area A1*. A1* is equal to that of the free solvent peak A2* in magnitude but opposite in sign. In this study the data of A3eff, A3 and A2* were measured for the system of polystyrene(3)-chloroform(1)-methanol(2). The results support the analytical prediction and indicate that the preferential adsorption coefficient can be determined from either the polymer peak or the free solvent peak.

Key words: Size-exclusion chromatography, Preferential solvation, Solvent mixture

中图分类号: 

TrendMD: