高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒的制备及其性质研究

何晓晓1,2,3,4, 陈基耘1,2,4, 王柯敏1,2,4, 谭蔚泓1,2,4, 秦迪岚1,2,4   

    1. 湖南大学化学生物传感与计量学国家重点实验室,
    2. 生物医学工程中心,
    3. 生命科学与技术研究院,
    4. 湖南省生物纳米工程技术研究中心, 长沙 410082
  • 收稿日期:2006-01-10 修回日期:1900-01-01 出版日期:2006-10-10 发布日期:2006-10-10
  • 通讯作者: 王柯敏

Preparation and Properties of Cy3 Doped Core-shell Silica Fluorescent Nanoparticals

HE Xiao-Xiao1,2,3,4, CHEN Ji-Yun1,2,4, WANG Ke-Min1,2,4, TAN Wei-Hong1,2,4, QIN Di-Lan1,2,4   

    1. State Key Laboratory of Chemo/Biosensing and Chemometrics,
    2. Engineering Center for Biomedicine,
    3. Institute of Life Science & Biological Technology,
    4. Engineering Research Center for Bio-Nanotechnology of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2006-01-10 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
  • Contact: WANG Ke-Min

PDF (PC)

被引次数

50

摘要： 以蛋白质或多肽修饰的吲哚类菁染料Cy3为内核, 采用实验条件简单的油包水反相微乳液方法成核, 通过正硅酸乙酯水解形成的网状二氧化硅包壳的方法制备吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒. 考察了以不同等电点的蛋白质和多肽修饰的Cy3为内核材料对吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒制备的影响. 结果表明, 分别采用人免疫球蛋白(IgG)或多聚赖氨酸修饰的Cy3为内核材料, 都能制备荧光强度高、荧光稳定性强和染料泄漏极少的Cy3嵌入的核壳荧光纳米颗粒. 进一步
对Cy3嵌入的核壳荧光纳米颗粒进行了表征, 并将基于这一新型的荧光纳米颗粒建立起来的生物标记方法初步应用于流感病毒DNA的检测, 其检测线性范围为3.18×10-10～1.27×10-9 mol/L, 检测下限为3.51×10-10 mol/L, 相关系数r为0.986 5.

关键词: 吲哚类菁染料Cy3, 油包水微乳液, 核壳荧光纳米颗粒, DNA检测

Abstract: Cy3 dye doped core-shell silica fluorescent nanoparticles was synthesized by using of water-in-oil microemulsion technique, with biomolecules modified Cy3 as the core and the silica produced from the hydrolysis TEOS(tetraethyl orthosilicate) as the shell. In this paper, five kinds of bimolecules with different pI values for preparation of Cy3 doped core-shell silica fluorescent nanoparticles were investigated. The results show that very bright and photostable Cy3 doped core-shell silica fluorescent nanoparticles can be both prepared by using Cy3-IgG or Cy3-polysine modified as the core material. And then Cy3-IgG was selected as a demonstration to prepare Cy3 doped core-shell silica fluorescent nanoparticles. A novel fluorescence marker was based by using the Cy3 doped core-shell silica fluorescent nanoparticles. The flu virus DNA was detected by using the Cy3 doped core-shell silica fluorescent nanoparticles, and the detection limit of the target DNA is 3.51×10-10 mol/L.

Key words: Cy3 dye, Water-in-oil microemulsion, Core-shell fluorescent nanoparticals, DNA detection

中图分类号: 

TrendMD: