高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

利用GC/MRM检测去势大鼠前列腺中睾酮、双氢睾酮和脱氢表雄酮

陈君1, 梁琼麟1, 李珲2, 罗国安1, 王义明1   

    1. 清华大学化学系, 北京 100084;
    2. 北京大学泌尿外科研究所, 北京大学第一医院, 北京 100034
  • 收稿日期:2008-04-02 修回日期:1900-01-01 出版日期:2008-11-10 发布日期:2008-11-10
  • 通讯作者: 罗国安

Detection of Testosterone, Dihydrotestosterone, Dehydroepiandrosterone in Emasculated Rat Prostate via GC/MRM

CHEN Jun1, LIANG Qiong-Lin1, LI Hui2, LUO Guo-An1*, WANG Yi-Ming1   

    1. Department of Chemistry, Tsinghua University, Beijing 100084, China;
    2. Institute of Urology, The First Hospital of Peking University, Peking University, Beijing 100034, China
  • Received:2008-04-02 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10
  • Contact: LUO Guo-An

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被引次数

7

摘要： 建立了一种分析睾酮、双氢睾酮及脱氢表雄酮的GC/MRM 方法. 该方法回收率高, 灵敏度好, 对样品需求小, 已运用于研究去势大鼠前列腺中各种雄激素含量的变化. 以去势大鼠为模型, 对去势后3个月内其前列腺内雄激素含量的变化进行检测分析. 去势后, 大鼠前列腺中雄激素含量经过2个月的平台后, 至术后3个月出现明显降低. 而抑制5α还原酶药物的作用, 术后2个月时能明显改变前列腺中激素含量水平.

关键词: 前列腺, 雄激素, 气相色谱-质谱联用

Abstract: Prostate cancer is a common malignancy in men. For prostate dependent on androgen for growth, orchiectomy is adopted in clinical therapy along with medicines aimed to restrain the synthesis and secretion of androgens in adrenal. The function of adrenal androgen is not clear till now, therefore, it needs more consideration on how to operate the therapy properly. We established a GC/MRM method for the detection of androgens in tissue sample, by which we monitored the concentration of androgens in emasculated rat. It is shown that the influence of adrenal androgen on the hormone metabolism in prostate existed via this experiment.

Key words: Prostate, Androgen, Gas chromatography-Mass spectrometry

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