高等学校化学学报

高等学校化学学报

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Co@SiO2核壳式纳米磁性粒子的合成、性质表征及在细胞分离和细胞芯片上的应用

郭小英1, 王永宁2, 顾林岗3, 贺艳峰4, 张春秀1, 唐祖明1, 陆祖宏1
   

    1. 东南大学生物科学与医学工程系, 吴健雄生物分子与电子学国家重点实验室, 南京 10096;
    2. 中国科学院理化技术研究所, 北京 100080;
    3. 江苏省公安厅物证鉴定中心, 南京 210024;
    4. 东南大学公共卫生学院, 南京 210009
  • 收稿日期:2005-09-21 修回日期:1900-01-01 出版日期:2006-09-10 发布日期:2006-09-10
  • 通讯作者: 陆祖宏

Synthesis Property Characterization of Coreshell Co@SiO2 Magnetic Nanoparticle and Its Biomedical Applications to Cell Separation and Cell Chip

GUO Xiao-Ying1, WANG Yong-Ning2, GU Lin-Gang3, HE Yan-Feng4, ZHANG Chun-Xiu1, TANG Zu-Ming1, LU Zu-Hong1
   

    1. State Key Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, China;
    2. Technical Institute of Physical and Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
    3. Division of the Criminal Investigation, Department of Jiangsu Provincial Public Security, Nanjing 210024, China;
    4. College of Public Health, Southeast University, Nanjing 210009, China
  • Received:2005-09-21 Revised:1900-01-01 Online:2006-09-10 Published:2006-09-10
  • Contact: LU Zu-Hong

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摘要： 合成了Co@SiO2核壳式纳米粒子, 并采用透射电镜(TEM)、X射线衍射(XRD)、扫描电镜(SEM)和振动样品磁强计(VSM)对其形状、尺寸、荧光及磁特性进行了表征, 探讨了其在细胞分离和细胞芯片上的应用和原理.

关键词: 核壳式, 纳米磁珠, 细胞芯片, 细胞分离

Abstract: In this paper, Co@SiO2 nanoparticles with the apparent coreshell magnetic nanostructure were synthesized by reducing and hydrolysis in aqueous solutions. The TEM, VSM, SEM, and the confocal fluorescence microscope were utilized to characterize the morphology, size, magnetism of the Co@SiO2 nanoparticles, and the coreshell materials were applied to the cell separation, immunophenotyping of leukemias and lymphomas diagnostics chip, in which strenuous procedures were omitted by the use of the magnetic coreshell nanoparticles, and separation. These core-shell nanoparticles were proved to have obvious cores and shell, and the cores have strong magnetism, and the shell protected these nanosized metal particles from coagulation due to anistropic dipolar attraction and oxidation very well. However this method could not limited the original cores below the size of 10 nm, which is the critical size of superparamagnetisizm, as a result coagulation due to remanent magnetism limited this core-shell particles from further applications in bioseparation.

Key words: Core-shell, Magnetic nanoparticle, Immunophenotyping of leukemias and lymphomas, Cell separation

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