白骨壤酸性果胶多糖HAM-3-Ⅱb-Ⅱ的结构分析

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (3): 492.

白骨壤酸性果胶多糖HAM-3-Ⅱb-Ⅱ的结构分析

方旭波¹, 江波², 陈小娥¹

1. 浙江海洋学院食品与药学学院, 舟山 316004;
2. 江南大学食品科学与技术国家重点实验室, 无锡 214122

收稿日期:2009-06-30 出版日期:2010-03-10 发布日期:2010-03-10
通讯作者: 方旭波, 男, 博士, 副教授, 主要从事天然产物资源的研究. E-mail: fxb70@sohu.com
基金资助:
浙江省教育厅项目(批准号: 20070418)资助.

Structure Identification of an Acidic Pectin HAM-3-Ⅱb-Ⅱ from Avicennia Marina (Forsk.) Vierh.

FANG Xu-Bo^1,2*, JIANG Bo², CHEN Xiao-E¹

1. School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, China;
2. State Key Laboratory of Food Science and Technology, Southern Yangtze University, Wuxi 214122, China

Received:2009-06-30 Online:2010-03-10 Published:2010-03-10
Contact: FANG Xu-Bo. E-mail: fxb70@sohu.com
Supported by:
浙江省教育厅项目(批准号: 20070418)资助.

摘要/Abstract

摘要：

以分离纯化得到的白骨壤酸性多糖HAM-3-Ⅱb-Ⅱ为研究对象, 采用高碘酸氧化-Smith降解、部分酸水解以及甲基化分析等技术对该多糖的结构进行分析. 结果表明, HAM-3-Ⅱb-Ⅱ为典型的鼠李半乳糖醛酸聚糖I 型酸性果胶类多糖, 主链骨架包括: 1,4-连接的α-D-GalpA构成的无分支的半乳糖醛酸聚糖(光滑区)和通过α-D-GalpA的O4位与1,2-和1,2,4-L-Rhap的O2交替相连构成的含有较多分支的鼠李半乳糖醛酸聚糖(毛发区). 由T-、1,6-、1,3,6-、1,4-、1,4,6-D-Galp和T-、1,2-、1,3-、1,5-、1,2,5-、1,3,5-Aaraf聚合成的AGⅠ型阿拉伯半乳聚糖、AGⅡ型阿拉伯半乳聚糖、半乳聚糖以及阿拉伯聚糖, 构成了HAM-3-Ⅱb-Ⅱ的侧链部分, 通过Rha残基的O4位与主链相连.

关键词: 白骨壤; 果胶; 多糖; 结构分析; 甲基化

Abstract:

Avicennia marina(Forsk.) Vierh. is one of the most widespread mangroves species of the world. The plant is widely used in the treatment of skin disease, ailments and wounds. Researchers have so far focused their attentions on its medicinal functions and chemical constituents. HAM-3-Ⅱb-Ⅱ, an acidic polysaccharides was obtained from Avicennia Marina by a series step of extraction, isolation and purification. The structure was studied by periodate oxidation, Smith degradation, partial hydrolysis and methylation analysis. The results show that HAM-3-Ⅱb-Ⅱ is an acidic pectin-type belong to RGI family, the backbone of main chain contained homogalacturonan fragments as “smooth regions” with 1,4-α-D-GalpA and rhamnogalacturonan segments as “hairy regions” with repeating unit of →4)-α-D-GalpA-(1→2)-α-L-Rhap-(1. T-, 1,6-, 1,3,6-, 1,4-, 1,4,6-D-Galp and T-, 1,2-, 1,3-, 1,5-, 1,2,5-, 1,3,5-Araf are linked as arabinogalactan type Ⅰ, arabinogalactan type Ⅱ, galactan and arabinan at the cite of O4 of Rha in the form of the side chain. Hence, HAM-3-Ⅱb-Ⅱ was considered a new bioactive pectin from Avicennia Marina.

Key words: Avicennia marina (Forsk.) Vierh.; Pectin; Polysaccharide; Structure identification; Methylation analysis

TrendMD:

方旭波, 江波, 陈小娥. 白骨壤酸性果胶多糖HAM-3-Ⅱb-Ⅱ的结构分析. 高等学校化学学报, 2010, 31(3): 492.

FANG Xu-Bo*, JIANG Bo, CHEN Xiao-E. Structure Identification of an Acidic Pectin HAM-3-Ⅱb-Ⅱ from Avicennia Marina (Forsk.) Vierh.. Chem. J. Chinese Universities, 2010, 31(3): 492.

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