蚕沙叶绿素锌配合物对DNA的光断裂作用

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (3): 442.

蚕沙叶绿素锌配合物对DNA的光断裂作用

司利平¹, 刘晶晶¹, 史蕾¹, 王湘利¹, 张雷¹, 刘海洋¹, 计亮年²

1. 华南理工大学化学与化工学院, 广州 510641;
2. 中山大学化学与化学工程学院, 广州 510275

收稿日期:2009-07-26 出版日期:2010-03-10 发布日期:2010-03-10
通讯作者: 刘海洋, 男, 副教授, 主要从事大环化合物与化学生物学方面的研究. E-mail: chhyliu@scut.edu.cn
基金资助:
国家自然科学基金(批准号: 20771039, 20871122, 20971046)资助.

Effect of Zn-Chlorophyll Prepared from Silkworm Faeces on DNA Photocleavage

SI Li-Ping¹, LIU Jing-Jing¹, SHI Lei¹, WANG Xiang-Li¹, ZHANG Lei¹, LIU Hai-Yang^1*, JI Liang-Nian²

1. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China;
2. School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China

Received:2009-07-26 Online:2010-03-10 Published:2010-03-10
Contact: LIU Hai-Yang. E-mail: chhyliu@scut.edu.cn
Supported by:
国家自然科学基金(批准号: 20771039, 20871122, 20971046)资助.

摘要/Abstract

摘要：

利用从蚕沙中提取的叶绿素(CHL)制得了锌叶绿素Z(n-CHL)配合物. 采用紫外-可见光谱、CD光谱和黏度法研究了Zn-CHL与小牛胸腺DNA(ct-DNA)的相互作用, 并采用琼脂糖凝胶电泳研究了光照条件下Zn-CHL对pBR322 DNA的切割能力. 结果表明, Zn-CHL与ct-DNA的作用方式属于外部结合模式, 其结合常数为 2.32×10⁵ L/mol, Zn-CHL在可见光照射下具有切割DNA的能力.

关键词: 蚕沙; 叶绿素; DNA; 光断裂

Abstract:

Zinc chlorophyll(Zn-CHL) complex was prepared using chlorophyll extacted from silkworm faeces. Relative viscosity, UV-Visible and CD spectra were used to study the interaction between Zn-CHL and ct-DNA. Agarose gel electrophoresis was also carried out to investigate the photocleavage ability of Zn-CHL to pBR 322 DNA. The results suggested that the binding mode of Zn-CHL and ct-DNA was outside binding, the binding constant was 2.32×10⁵ L/mol. Agarose gel electrophoresis picture showed that Zn-CHL could induce DNA cleavage upon irradiation.

Key words: Chlorophyll; Silkworm faeces; DNA; Photocleavage

TrendMD:

司利平, 刘晶晶, 史蕾, 王湘利, 张雷, 刘海洋, 计亮年. 蚕沙叶绿素锌配合物对DNA的光断裂作用. 高等学校化学学报, 2010, 31(3): 442.

SI Li-Ping, LIU Jing-Jing, SHI Lei, WANG Xiang-Li, ZHANG Lei, LIU Hai-Yang. Effect of Zn-Chlorophyll Prepared from Silkworm Faeces on DNA Photocleavage. Chem. J. Chinese Universities, 2010, 31(3): 442.

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