黄柏生物碱成分在二妙丸类方中保护尿酸盐致人血管内皮细胞损伤作用及其配伍变化的研究

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (8): 1522.

黄柏生物碱成分在二妙丸类方中保护尿酸盐致人血管内皮细胞损伤作用及其配伍变化的研究

尹莲^1,2, 邓海山¹, 朱晓勤¹, 李欣¹, 段金廒², 丁安伟^1,2

1. 南京中医药大学药学院,
2. 江苏省方剂研究重点实验室, 南京 210046

收稿日期:2008-11-28 出版日期:2009-08-10 发布日期:2009-08-10
通讯作者: 尹莲, 女, 博士, 副教授, 主要从事中药复方物质基础及配伍规律的研究. E-mail: Yinlian162@163.com
基金资助:
江苏省自然科学基金(批准号: BK2002033)、江苏省方剂研究重点实验室开放项目(批准号: 022021014010)和南京中医药大学基础研究与重点培育项目(批准号: 08XPY04)资助.

Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula

YIN Lian^1,2*, DENG Hai-Shan¹, ZHU Xiao-Qin¹, LI Xin¹, DUAN Jin-Ao², DING An-Wei¹

1. Pharmacological College,
2. Key Laboratory for Modern Research of Traditional Chinese Medical Formula of Jiangsu, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China

Received:2008-11-28 Online:2009-08-10 Published:2009-08-10
Contact: YIN Lian. E-mail: Yinlian162@163.com
Supported by:
江苏省自然科学基金(批准号: BK2002033)、江苏省方剂研究重点实验室开放项目(批准号: 022021014010)和南京中医药大学基础研究与重点培育项目(批准号: 08XPY04)资助.

摘要/Abstract

摘要：

研究对二妙丸类方(二妙丸、三妙丸、四妙丸和加味四妙丸)起重要作用的基本方二妙丸的物质基础及其配伍变化. 正交组合二妙丸类方组方药有效部位, 四氮唑盐(MTT)法评价优选出对保护尿酸钠结晶(MSU)致人血管内皮细胞(HUVEC)损伤起重要作用的二妙丸黄柏生物碱部位, HPLC/DVD法建立黄柏生物碱部位指纹图谱, HPLC-ESI-MS/MS分析共有峰成分, 鉴定了12个生物碱成分, 并比较了二妙丸类方有效部位群HPLC/DVD指纹图谱配伍类方前后黄柏生物碱共有峰相对峰面积比值的变化. 结果表明, 黄柏生物碱配伍二妙丸及其类方后总相对含量分别为122.9%, 80.7%, 60.2%和94.2%, 小檗碱、巴马汀等重要生物碱也呈现较大的变化趋势, 茵芋碱、5-羟基小檗碱和四氢巴马汀等成分配伍二妙丸类方后消失. 研究显示, 基本方二妙丸中的黄柏生物碱是二妙丸类方中保护HUVEC起重要作用的物质基础, 其相对含量变化与配伍类方后的功效变化一致.

关键词: 二妙丸类方; 生物碱; 指纹图谱; 配伍; 液质联用

Abstract:

To reveal the compatibility of traditional Chinese medicine, the material foundation playing major role of Ermiao Wan categorized formula and their compatibility content variation was studied. The activity of the combined effective components by orthogonal design in its relevant herbs were evaluated for protecting HUVEC damaged model induced by urate, the results indicated that the alkaloids components of Phellodendron chinense(PC) of Ermiao Wan play a major role in its categorized formula. The fingerprint of alkaloids components in PC and Ermiao Wan categorized formula were analyzed and compared by high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS). Twelve alkaloids were detected in the fingerprint of alkaloids. The contents of some alkaloids were found to be reduced, on the contrary, the contents of total alkaloids were increased 122.9% in Ermiao Wan, and reduced 80.7%, 60.2%, 94.2% in its categorized formula. From the chemical point of view, the material foundation of basic formula plays major role in its categorized formula, the variation of relative content of alkaloids are in accord with the virtue of Ermiao Wan categorized formula.

Key words: Ermiao Wan categorized formula; Alkaloid; Fingerprint; Compatibility; HPLC-ESI-MS/MS

TrendMD:

尹莲, 邓海山, 朱晓勤, 李欣, 段金廒, 丁安伟 . 黄柏生物碱成分在二妙丸类方中保护尿酸盐致人血管内皮细胞损伤作用及其配伍变化的研究. 高等学校化学学报, 2009, 30(8): 1522.

YIN Lian*, DENG Hai-Shan, ZHU Xiao-Qin, LI Xin, DUAN Jin-Ao, DING An-Wei. Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula. Chem. J. Chinese Universities, 2009, 30(8): 1522.

参考文献

[1]LIU Ping(刘屏), WANG Jin-Liang(汪进良), WANG Yan(王燕), et al.. China Journal of Traditional Chinese Medicine and Pharmacyz(中华中医药杂志)[J], 2005, 20(5): 279—281
[2]LI Jing-Hua(李敬华), CUI Meng(崔蒙). Chinese Journal of Information on Traditional Chinese Medicine(中国中医药信息杂志)[J], 2007, 14(5): 104—105
[3]LI Guo(李果), XIAO Xiao-He(肖小河), JIN Cheng(金城), et al.. Chinese Journal of Clincal Rehabililation(中国临床康复)[J], 2006, 10(43): 174—176
[4]HE Wei(何伟), QIN Lin(秦林), SI Shu-Yuan(司淑媛).Traditional Chinese Medicine(中草药)[J], 2002, 33(7): 600—602
[5]CHEN Jian-Ping(陈建萍), TAN Bing-Yan(谭炳炎), WU Wei-Kang(吴伟康), et al.. Chinese Journal of Experimen Traditional Medical Formulae(中国实验方剂学杂志)[J], 2001, 7(3): 16—17
[6]ZHU Dan-Ni(朱丹妮), YAN Yong-Qing(严永清), LI Zhi-Ming(李志明). China Journal of Chinese Material Medica(中国中药杂志)[J], 1998, 23(8): 483—485
[7]XIA Yun(夏云), LI Zhi-Ming(李志明), ZHU Dan-Ni(朱丹妮). China Journal of Chinese Material Medica(中国中药杂志)[J], 1998, 23(4): 230—232
[8]LI Xiu-Ling(李秀玲), XIAO Hong-Bin(肖红斌), XU Qing(徐青), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2004, 25(9): 1632—1636
[9]HUANG Hao(黄浩), ZHOU Lin(周琳), JIANG Biao(姜标). World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中医药现代化)[J], 2005, 7(4): 18—22
[10]YIN Lian(尹莲), SHI Xin-De(史欣德). World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中药现代化)[J], 2006, 8(6): 27—30
[11]TAN Qiu-Wei(谭秋薇), XU Li(徐立), YIN Lian(尹莲), et al.. China Applied Medical Magazine(中国实用医学杂志)[J], 2008, 3(4): 10—12
[12]ZHU Xiao-Qin(朱晓勤), YIN Lian(尹莲), XU Li(徐立), et al.. Chinese Archives of Traditional Chinese Medicine(中医药学导报)[J], 2008, 14(2): 12—15
[13]ZHU Xiao-Qin(朱晓勤). Study on Correlation of Material Foundation and Biological Effect on Anti-Damp-Heat-Syndrome Gout of Ermiao Wan Categorized Formula(二妙丸类方抗湿热证痛风物质基础与生物效应的相关性研究)[D], Nanjing: Nanjing University of Traditional Chinese Medicine, 2008: 45—50
[14]YIN Lian(尹莲), XU Li(徐立), SHI Le(时乐). World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中药现代化)[J], 2005, 7(4): 28—33
[15]LIAO Rong-Xin(廖荣鑫), WU Shi-Jiu(吴仕九), WEN Xiao-Min(文小敏). Journal of Traditional Chinese Medicine(辽宁中医杂志)[J], 2005, 32(10): 1007—1009
[16]Couser W. G.. J. Am. Soc. Nephrol[J], 1990, 1: 13—17
[17]WANG Heng-Qi(王衡奇), QIN Min-Jian(秦民坚), YU Guo-Dian(余国奠). Chinese Wild Plant Resouces(中药野生植物资源)[J], 2001, 20(4): 4—8
[18]CHEN Ting(陈婷), XU Qiang(徐强), YAN Yong-Qing(严永清), et al.. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学)[J], 1995, 1(2): 20—22
[19]CHEN Han-Ying(陈韩英), LIU Li-Mei(刘丽梅), XU Jian-Qin(许剑琴), et al.. Veterinary Science in China(中国兽医科学)[J], 2007, 37(7): 602—605
[20]QIAN Jun(钱俊), YIN Lian(尹莲). World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中药现代化)[J], 2007, 9(1): 40—45
[21]PAN Yang(潘扬), WANG Tian-Shan(王天山), MA Guo-Xiang(马国祥), et al.. Traditional Chinese Medicine(中草药)[J], 1997, 28(3): 142—144
[22]Ikuta A., Nakamura T., Vrabe H.. Phytochemistry[J], 1998, 48(2): 285—291
[23]Ida Y., Satoh Y., Ontsuka M.. Phytochemistry[J], 1994, 35(1): 209—215
[24]Ikuta A., Vrabe H., Nakamura T.. J. Nat. Prod.[J], 1998, 61(8): 1012—1014
[25]HE Xian-Hui(何贤辉), ZENG Yao-Ying(曾耀英), XU Li-Hui(徐丽慧), et al.. Chinese Journal of Pathophysiology(中国病理生理杂志)[J], 2002, 18(10): 1183—1186